Recognizes alpha-smooth-muscle isoform of actin (Mr 43000). Reactivities on cultured stress fibers of smooth muscle-derived cells. Tumors specifically detected: Leiomyosarcoma, leiomyoma, and certain stromal cells surrounding infiltrating ductal carcinoma of breast. Species cross-reactivity: human, bovine, equine, rat, mouse, chicken.
Purification
Protein A purified
Immunogen
Synthetic N-terminus decapeptide of alpha-smooth-muscle isoform of actin.
Usage: Suitable for use in Immunofluorescence, Western Blot and Immunohistochemistry (frozen and paraffin-embedded tissue as well as cytological material). Immunohistochemistry: Ready-to-use on frozen sections. Paraffin embedded sections require protease pretreatment prior to antibody application. Incubate 1 hour at RT, extended with paraffin.
Restrictions
For Research Use only
Format
Liquid
Concentration
Lot specific
Buffer
PBS, pH 7.4, 0.5 % BSA, 0.09 % sodium azide.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.