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CD59 antibody (FITC)

CD59 Reactivity: Human FACS, IF Host: Mouse Monoclonal FITC

Catalog No. ABIN6735023

Datasheet as PDF

Target See all CD59 Antibodies

CD59
Reactivity
145

14

9

8

5

3

2

1

1
Human
Host
88

76

6

6
Mouse
Compatible Secondaries
Clonality
97

76

1
Monoclonal
Conjugate
83

20

14

14

6

4

2

2

2

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1
This CD59 antibody is conjugated to FITC
Application
101

72

49

40

35

28

26

17

14

14

13

13

11

11

8

3

3

3

1

1

1
Flow Cytometry (FACS), Immunofluorescence (IF)

Specificity

Binds to a 17-25kD glycoprotein, which is distributed on human cells and has a glycosyl-phosphatidylinositol (GPI) linkage to the plasma membrane. Cell reactivity: Recognizes all leucocytes, platelets, fibroblasts, erythrocytes and most cell lines. It is negative on U937 and Daudi cell lines. It is reduced on affected cells from patients with PNH with a distribution reflecting the clonal nature of the disease.

Purification

Purified

Immunogen

Human erythrocytes.

Type of Immunogen: Cells

Isotype

IgG1

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CD59 Reactivity: Human WB, FACS, IF, IHC (p) Host: Rabbit Polyclonal RB56691 unconjugated

anti-CD59 (CD59) antibody
CD59 Reactivity: Human FACS, IP, IHC (p) Host: Mouse Monoclonal MEM-43 unconjugated

anti-CD59 (CD59) antibody
CD59 Reactivity: Human, Mouse WB, FACS, IP, IHC (p) Host: Mouse Monoclonal MEM-43-5 unconjugated

anti-CD59 (CD59) (AA 31-111) antibody
CD59 Reactivity: Human WB, FACS, IHC, ELISA, IF/ICC Host: Mouse Monoclonal unconjugated

anti-CD59 (CD59) (AA 23-105) antibody
CD59 Reactivity: Rat WB, IHC, IP, ICC Host: Rabbit Polyclonal unconjugated

anti-CD59 (CD59) (AA 1-128) antibody
CD59 Reactivity: Human WB, ELISA Host: Mouse Monoclonal 3G6 unconjugated

anti-CD59 (CD59) (AA 26-102) antibody
CD59 Reactivity: Human WB, IHC, ELISA, IF Host: Rabbit Polyclonal unconjugated

anti-CD59 (CD59) (AA 52-100) antibody
CD59 Reactivity: Human FACS, ELISA, IHC (p), IHC (fro), IF (cc), IF (p) Host: Rabbit Polyclonal unconjugated

anti-CD59 (CD59) antibody
CD59 Reactivity: Human WB, IHC, ELISA Host: Mouse Monoclonal 2E11B6 unconjugated

Application Notes

Approved: Flo, IF (1:25)

Usage: Studies of complement and homologous restriction of complement action of the properties of GPI linked proteins and in the characterization of cells from patients with PNH. Flow cytometry and indirect immunofluorescence: 1:25. Dilute with isotonic buffer. Use 50 μL of the appropriate dilution per 1x1 x10⁶ in 100 μL buffer. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Comment

Target Species of Antibody: Human

Restrictions

For Research Use only
Format

Liquid

Concentration

Lot specific

Buffer

PBS,  pH 7.4, 0.2 % BSA, 0.1 % sodium azide.

Preservative

Sodium azide

Precaution of Use

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage

4 °C,-20 °C

Storage Comment

Short term: 4°C
Long term: Add glycerol (40-50%) -20°C.
Target

CD59

Alternative Name

CD59 (CD59 Products)

Background

Name/Gene ID: CD59

Synonyms: CD59, 16.3A5, 1F5, 1F5 antigen, CD59 glycoprotein, EL32, EJ16, G344, HRF-20, MAC-IP, MACIF, Ly-6-like protein, MEM43, MIRL, MEM43 antigen, MIN2, MIN3, MSK21, p18-20, Protectin, MAC-inhibitory protein, MIC11, T cell-activating protein, CD59 antigen, EJ30, HRF20, Human leukocyte antigen MIC11, Lymphocytic antigen CD59/MEM43, MIN1

Gene ID

966

UniProt

P13987

Pathways

Complement System