Prokineticin 1 antibody (Biotin)

Catalog No. ABIN465207

Product Details anti-Prokineticin 1 Antibody (Biotin)

Target: Prokineticin 1 (Prok1)

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This Prokineticin 1 antibody is conjugated to Biotin

Application: Western Blotting (WB), ELISA

Specificity: Human EG-VEGF

Purification: Immunoaffinity purified

Immunogen: E.coli derived recombinant Human Human EG-VEGF.

Type of Immunogen: Cell extract

Application Details

Application Notes: Approved: ELISA (1 μg/mL), WB (0.1 - 0.2 μg/mL)

Usage: The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested. ELISA: To detect hEG-VEGF by direct ELISA (using 100 μL/well antibody solution) a concentration of 0.25-1 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant hEG-VEGF. To detect hEG-VEGF by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25-1 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with Polyclonal Anti-Human EG-VEGF (LS-C104481) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hEG-VEGF. Western Blot: To detect hEG-VEGF by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hEG-VEGF is 1.5-3 ng/lane, under either reducing or non-reducing conditions.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Sterile PBS containing 0.1 % BSA

Concentration: Lot specific

Buffer: Lyophilized from PBS,  pH 7.2

Handling Advice: Avoid freeze/thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Store Lyophilized at room temperature up to 1 month
Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.

Target Details for Prokineticin 1

Target: Prokineticin 1 (Prok1)

Alternative Name: PROK1 / EG-VEGF (Prok1 Products)

Background: Name/Gene ID: PROK1

Synonyms: PROK1, EG-VEGF, EGVEGF, Mambakine, PRK1, Prokineticin-1, PK1, Prokineticin 1

Gene ID: 84432

UniProt: P58294