TWEAK antibody

Catalog No. ABIN465065

Product Details anti-TWEAK Antibody

Target: TWEAK (TNFSF12) (Tumor Necrosis Factor (Ligand) Superfamily, Member 12 (TNFSF12))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This TWEAK antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Neutralization (Neut)

Specificity: Human TWEAK

Purification: Immunoaffinity purified

Immunogen: E.coli derived recombinant Human TWEAK.

Type of Immunogen: Cell extract

Application Details

Application Notes: Approved: ELISA (0.5 - 2 μg/mL), Neut (0.1 - 0.2 μg/mL), WB

Usage: Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of hTWEAK (200 ng/mL), a concentration of 0.1-0.2 μg/mL of this antibody is required. ELISA: To detect hTWEAK by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5-2 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human TWEAK (LS-C104282) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hTWEAK. Western Blot: To detect hTWEAK by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hTWEAK is 1.5-3 ng/lane, under either reducing or or non-reducing conditions.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Sterile water

Concentration: Lot specific

Buffer: Lyophilized from PBS,  pH 7.2

Handling Advice: Avoid freeze/thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Store Lyophilized at room temperature up to 1 month
Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.

Target Details for TWEAK

Target: TWEAK (TNFSF12) (Tumor Necrosis Factor (Ligand) Superfamily, Member 12 (TNFSF12))

Alternative Name: TNFSF12 / TWEAK (TNFSF12 Products)

Background: Name/Gene ID: TNFSF12
Family: TNF

Synonyms: TNFSF12, APO3/DR3 ligand, APO3L, DR3LG, APO3 ligand, TWEAK

Gene ID: 8742

UniProt: O43508

Pathways: Apoptosis