Glutathione Peroxidase 1 antibody (AA 130-142)

Catalog No. ABIN461936

Product Details anti-Glutathione Peroxidase 1 Antibody

Target: Glutathione Peroxidase 1 (GPX1)

Binding Specificity: AA 130-142

Reactivity: Human, Monkey, Horse

Host: Goat

Clonality: Polyclonal

Conjugate: This Glutathione Peroxidase 1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Brand: IHC-plus™

Specificity: Human GPX1. This antibody is expected to recognise isoform 1 ( NP_000572.2) only.

Predicted Reactivity: Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Elephant, Horse (100%) Panda, Bat, Pig (92%) Rat, Hamster, Zebu, Bovine, Dog, Rabbit (85%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide C-REALPAPSDDATA from an internal region of human GPX1 (NP_000572.2). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Elephant, Horse (100%), Panda, Bat, Pig (92%), Rat, Hamster, Zebu, Bovine, Dog, Rabbit (85%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (3.75 μg/mL), Peptide-ELISA (1:32000), WB (1 - 3 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 3.75 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for Glutathione Peroxidase 1

Target: Glutathione Peroxidase 1 (GPX1)

Alternative Name: GPX1 / Glutathione Peroxidase (GPX1 Products)

Background: Name/Gene ID: GPX1

Synonyms: GPX1, Glutathione peroxidase, GSHPx-1, Glutathione peroxidase 1, GPx-1, GSHPX1, GPXD

Gene ID: 2876

NCBI Accession: NP_000572

UniProt: P07203

Pathways: Thyroid Hormone Synthesis, Sensory Perception of Sound, Skeletal Muscle Fiber Development, Cell RedoxHomeostasis, Negative Regulation of intrinsic apoptotic Signaling, SARS-CoV-2 Protein Interactome