Human Papilloma Virus 18 E2 (HPV-18 E2) (AA 1-83) antibody

Catalog No. ABIN457548

Product Details

Target: Human Papilloma Virus 18 E2 (HPV-18 E2)

Binding Specificity: AA 1-83

Reactivity: Human Papillomavirus (HPV)

Host: Mouse

Clonality: Monoclonal

Conjugate: Un-conjugated

Application: ELISA, Western Blotting (WB), Immunofluorescence (IF)

Specificity: Reacts with HPV-18 E2-protein.

Purification: purified with Protein G affinity chromatorgaphy

Immunogen: Recombinant full-length HPV-18 E2-protein purifiedfrom E. coli

Clone: 2E7

Isotype: IgG

Application Details

Application Notes: ELISA:1: 2 000, WB: 1: 1000. This antibody does not work in IP. Monoclonal antibody working titer has to be established practically for each particular antigen and assay format

Restrictions: For Research Use only

Independent Validation (UP)

Validation #102723 (Unfolding Profile)

by: NanoTemper Technologies

No.: #102723

Date: 11/05/2018

Antigen: HPV-18

Lot Number: A10P2E71-001

Method validated: Unfolding Profile

Positive Control: ABIN457548

Notes:

Passed. ABIN457548 showed a clear unfolding profile with two detected unfolding events with T_is at 78.4°C and 84.4°C. It is not unusual for antibodies to show more than one unfolding transition, as they are composed of distinct domains that may unfold independently and at different temperatures. This result suggests that the protein is properly folded and functional.

Validation Images

Unfolding profile of ABIN457548. The fluorescence signal is plotted against temperature. The native (folded) protein has a low signal at the beginning of the experiment, which increases upon unfolding, showing two unfolding events. The vertical lines indicate the Tis at 78.4 °C and 84.4 °C.

Full Methods

Primary Antibody: ABIN457548

Full Protocol:

• Dilute ABIN457548 1:10 in PBS buffer (Roth, 1058.1, lot 285231988) to get a final volume of 15µl at a concentration of 0.67µM.
• Load sample into Tycho capillary (NanoTemper Technologies, TY-C001).
• Run Tycho measurement.

Experimental Notes:

Tycho is designed to run quick and precise protein quality check experiments. Tycho uses intrinsic protein fluorescence to follow protein unfolding while running a fast thermal ramp, yielding results in 3min. A protein’s unfolding behavior is characterized by various parameters, most notably the inflection temperature (T_i). The T_i can be used to identify properly folded protein, to compare different batches, or to analyze the influence of storage/transport conditions on a protein. An absence of T_i would suggest that the protein is already unfolded and therefore most likely nonfunctional.

Handling

Format: Liquid

Concentration: 1 mg/ml

Buffer: PBS pH 7.4, with 0.1%

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Divide antibody into aliquots prior usage. Avoid multiple freeze-thaw cycles.

Storage: -20 °C

Storage Comment: This product is shipped in non-frozen liquid form in ambient conditions

Target Details

Target: Human Papilloma Virus 18 E2 (HPV-18 E2)

Target Type: Viral Protein

Background: Human papillomaviruses(HPV) are small DNA viruses whichinfect epithelia of the skin and mucosa. Over 90 types have been identified andthey mostly cause a variety of benign lesions such as warts and verrucae.However, some subtypes, notably types 16 and 18, 31 and 33, have been confirmedas agents which cause cervical cancer. Human Papillomavirus (HPV) E7 proteins havecellular transforming and trans-activating activities