CD3 antibody

Catalog No. ABIN457398

Product Details anti-CD3 Antibody

Target: CD3

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD3 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Functional Studies (Func)

Specificity: The mouse monoclonal antibody OKT3 recognizes an extracellular epitope on CD3 antigen of the TCR/CD3 complex on mature human T cells. This antibody, also known as Orthoclone OKT3 or Muromonab-CD3, has been extensively used as a drug for therapy of acute, glucocorticoid resistant rejection of allogenic renal, heart and liver transplants. It has also been investigated for use in treating T-cell acute lymphoblastic leukemia.

Cross-Reactivity (Details): Human

Purification: Purified by protein-A affinity chromatography.

Purity: > 95 % (by SDS-PAGE)

Endotoxin Level: Endotoxin level is less than 0.01 EU/µg of the protein, as determined by the LAL test.

Immunogen: human T cells

Clone: OKT3

Isotype: IgG2a

Application Details

Application Notes: Functional application: counteracting both generation and function of effector T cells.
Flow cytometry: Recommended dilution: 1 μg/mL.

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4

Preservative: Azide free

Handling Advice: Do not freeze.

Storage: 4 °C

Storage Comment: Store at 2-8°C. Do not freeze.

References for anti-CD3 antibody (ABIN457398)

Southworth, Plumb, Gupta, Pearson, Ramis, Lehner, Miralpeix, Singh: "Anti-inflammatory potential of PI3Kδ and JAK inhibitors in asthma patients." in: Respiratory research, Vol. 17, Issue 1, pp. 124, (2017) (PubMed).

Li, Wong, Yi, Wong, Zhou, Liu et al.: "Matrine induces cell anergy in human Jurkat T cells through modulation of mitogen-activated protein kinases and nuclear factor of activated T-cells signaling with concomitant up-regulation of ..." in: Biological & pharmaceutical bulletin, Vol. 33, Issue 1, pp. 40-6, (2010) (PubMed).

Quintana, Schwindling, Wenning, Becherer, Rettig, Schwarz, Hoth: "T cell activation requires mitochondrial translocation to the immunological synapse." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 104, Issue 36, pp. 14418-23, (2007) (PubMed).

Trushin, Bren, Asin, Pennington, Paya, Badley: "Human immunodeficiency virus reactivation by phorbol esters or T-cell receptor ligation requires both PKCalpha and PKCtheta." in: Journal of virology, Vol. 79, Issue 15, pp. 9821-30, (2005) (PubMed).

Undale, van den Elsen, Celis: "Antigen-independent acquisition of MHC class II molecules by human T lymphocytes." in: International immunology, Vol. 16, Issue 10, pp. 1523-33, (2004) (PubMed).

Lang, Handgretinger, Niethammer, Schlegel, Schumm, Greil, Bader, Engel, Scheel-Walter, Eyrich, Klingebiel: "Transplantation of highly purified CD34+ progenitor cells from unrelated donors in pediatric leukemia." in: Blood, Vol. 101, Issue 4, pp. 1630-6, (2003) (PubMed).

Gordon, Leimig, Mueller, Babarin-Dorner, Holladay, Houston, Kerst, Geiger, Handgretinger: "A large-scale method for T cell depletion: towards graft engineering of mobilized peripheral blood stem cells." in: Bone marrow transplantation, Vol. 30, Issue 2, pp. 69-74, (2002) (PubMed).

Schlegel, Eyrich, Bader, Handgretinger, Lang, Niethammer, Klingebiel: "OKT-3-based reconditioning regimen for early graft failure in HLA-non-identical stem cell transplants." in: British journal of haematology, Vol. 111, Issue 2, pp. 668-73, (2001) (PubMed).

Smith: "Ten years of Orthoclone OKT3 (muromonab-CD3): a review." in: Journal of transplant coordination : official publication of the North American Transplant Coordinators Organization (NATCO), Vol. 6, Issue 3, pp. 109-19; quiz 120-1, (1997) (PubMed).

Target Details for CD3

Target: CD3

Alternative Name: CD3 (CD3 Products)

Background: CD3 antigen, epsilon polypeptide,CD3 complex is crucial in transducing antigen-recognition signals into the cytoplasm of T cells and in regulating the cell surface expression of the TCR complex. T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta. These CD3 subunits are structurally related members of the immunoglobulins super family encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules. This association is mediated at least in part by a double tyrosine-based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR-induced growth arrest, cell survival and proliferation. The CD3 antigen is present on 68-82 % of normal peripheral blood lymphocytes, 65-85 % of thymocytes and Purkynje cells in the cerebellum. It is never expressed on B or NK cells. Decreased percentages of T lymphocytes may be observed in some autoimmune diseases.,CD3E, T3E, TCRE

Gene ID: 916

UniProt: P07766

Pathways: TCR Signaling, Ubiquitin Proteasome Pathway