RNF34 antibody (N-Term)

Catalog No. ABIN453991

Product Details anti-RNF34 Antibody

Target: RNF34 (Ring Finger Protein 34 (RNF34))

Binding Specificity: N-Term

Reactivity: Human, Mouse, Rat, Cow, Dog

Host: Goat

Clonality: Polyclonal

Conjugate: This RNF34 antibody is un-conjugated

Application: Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Specificity: This antibody is expected to recognize both reported isoforms of Human RNF34 (NP_919247.1 and NP_079402.2).

Cross-Reactivity (Details): Species reactivity (expected):Mouse, Rat, Canine (Dog) and Cow.
Species reactivity (tested):Human.

Purification: Ammonium Sulphate Precipitation followed by antigen Affinity Chromatography using the immunizing peptide.

Immunogen: Peptide with sequence KAGATSMWASCC, from the N Terminus of the protein sequence according to NP_919247.1, NP_079402.2. Genename: RNF34

Application Details

Application Notes: Peptide ELISA: Limit Dilution: 1/32000. Immunohistochemistry: 3-5 μg/mL. In paraffin embedded Human Kidney shows strongstaining of cytoplasm in DCT.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH 7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

Target Details for RNF34

Target: RNF34 (Ring Finger Protein 34 (RNF34))

Alternative Name: RFI / RNF34 (RNF34 Products)

Background: RNF34, also known as RING finger protein 34 is a novel modulator of NF-kappaB activation. RNF34 possesses E3 ubiquitin protein ligase activity and has been shown to regulate the levels of CASP8 and CASP10 by targeting them for proteasomal degradation. RNF34 protects cells against apoptosis induced by TNF and may bind phosphatidylinositol phosphates. RNF34 is localized to both the nucleus and the cytoplasm. In the nucleus, a distinct speckled pattern is observed by immunofluorescence microscopy. In the cytoplasm, the protein is associated with membranes. RNF34 is expressed ubiquitously and can be detected in heart, brain, liver, skeletal muscle, kidney, pancreas, spleen, thymus, prostate, testis, ovary, colon and leukocytes. Multiple transcript variants have been reported for this protein.Synonyms: CARP-1, CARP1, Caspases-8 and -10-associated RING finger protein 1, E3 ubiquitin-protein ligase RNF34, FYVE-RING finger protein Momo, Human RING finger homologous to inhibitor of apoptosis protein, RIFF

Gene ID: 80196

NCBI Accession: NP_079402

UniProt: Q969K3