Helicobacter Pylori antibody

Catalog No. ABIN452543

Product Details anti-Helicobacter Pylori Antibody

Target: Helicobacter Pylori (H. pylori)

Reactivity: Helicobacter pylori

Host: Mouse

Clonality: Monoclonal

Conjugate: This Helicobacter Pylori antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA), ELISA (Capture)

Characteristics: Synonyms: H. pylori CagA Gene Product, Helicobacter pylori cytokine associated gene A

Purification: Affinity Chromatography on Protein G.

Immunogen: A highly immunogenic 32Kd CagA fragment, recombinant.

Clone: 1-00E-010

Isotype: IgG2a

Application Details

Application Notes: ELISA: HP CagA antibody (Clone 10E9) can be used as Capture antibody. In Sandwich ELISA, anti-HP CagA antibody clone 5H10 (Cat#ABIN452544) coated wellsmatches with HRP conjugated clone 5C6, clone 3C10 and clone 3C1 to detect therecombinant antigen. In addition, this clone selectively detected cell lysate of a CagA containing HP stain whenHRP conjugated clone 3C10 was used. Western Blotting: This clone detected a 32 KD band corresponding to the molecular weightof the recombinant CagA antigen.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore with Double distillated water to adjust the final concentration to 1.0 mg/mL.

Buffer: 0.01 M PBS, pH 7.0 without preservatives

Preservative: Without preservative

Storage: -20 °C

Storage Comment: Store the antibody at -20 °C. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for Helicobacter Pylori

Target: Helicobacter Pylori (H. pylori)

Alternative Name: Helicobacter Pylori (H. pylori Products)

Target Type: Virus

Background: Helicobacter pylori (H. pylori), a spiral rod shaped gram-negative bacterium, is frequently found in the stomach. In infected populations, 10-20 % may develop gastritis and gastric ulcer and 1-2 % may develop cancer. The genome of H. pylori isolates from carriers with symptoms contains a 40kb pathogenicity island encoding H. pylori cytotoxin, cytotoxin associated gene A protein (CagA) and other virulence associated factors. CagA is used as a biomarker for virulent H. pylori strains.Synonyms: H. pylori