HCV NS3 antibody

Catalog No. ABIN452520

Product Details anti-HCV NS3 Antibody

Target: HCV NS3 (Hepatitis C Virus NS3 (HCV NS3))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This HCV NS3 antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Cross-Reactivity (Details): Species reactivity (tested):Human.

Characteristics: Synonyms: Hepatitis C Virus Serine proteiase NS3

Purification: Affinity Chromatography on Protein G.

Immunogen: Purified Recombinant Chimeric HCV Polyprotein (555 a. a).

Clone: 281

Isotype: IgG1

Application Details

Application Notes: ELISA. Western Blot: Use of Hepatitis C Virus NS3 antibody (clone 281) at 0.1-1 μg/mL will allowvisualization of 100 ng/lane of both Recombinant Chimeric HCV Polyprotein andRecombinant NS-3 Protein. No reaction can be seen with Synthetic NS-3. Testing is underreducing and non-reducing conditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore with Double distillated water to adjust the final concentration to 1.0 mg/mL

Buffer: 0.01 M PBS, pH 7.2 without preservatives.

Preservative: Without preservative

Storage: -20 °C

Storage Comment: Store the antibody at -20 °C. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for HCV NS3

Target: HCV NS3 (Hepatitis C Virus NS3 (HCV NS3))

Alternative Name: HCV NS3 (HCV NS3 Products)

Target Type: Viral Protein

Background: HCV is a positive, single-stranded RNA virus in the Flaviviridae family. The genome is approximately 10,000 nucleotides and encodes a single polyprotein of about 3,000 amino acids. The polyprotein is processed by host cell and viral proteases into three major structural proteins including NS3, and several non-structural proteins necessary for viral replication. The NS3 part of the polyprotein displays three enzymatic activities: serine protease, NTPase and RNA helicase. The NS3 serine proteinase (NS3P) is a non-structural hepatitis C protein responsible for proteolytic processing of other non-structural proteins, because of this, it is also the most extensively studied protein of the Hepatitis C genome. It is responsible for proteolytic processing of the entire downstream region of the HC polyprotein, catalyzing cleavage at the NS3/NS4a, NS4a/NS4b, NS4b/NS5a, and NS5a/NS5b sites to release the mature NS3, NS4a, NS4b, NS5a, and NS5b proteins.For proper function, NS3 requires NS4a as a cofactor, but, interestingly enough, NS3 also cleaves the NS4a protein. The molecular weight of the monomer NS3P is 70 kDa.Synonyms: Hepatitis C Virus Serine proteiase NS3