HCV Core Protein antibody

Catalog No. ABIN452512

Product Details anti-HCV Core Protein Antibody

Target: HCV Core Protein (HCV C) (Hepatitis C Virus Core Protein (HCV C))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This HCV Core Protein antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Cross-Reactivity (Details): Species reactivity (tested):Human.

Characteristics: Synonyms: Hepatitis C Virus

Purification: Affinity Chromatography on Protein G.

Immunogen: Recombinant Chimeric Hepatitis C virus (HCV) polyprotein (555 amino acids).

Clone: C3

Isotype: IgG1

Application Details

Application Notes: ELISA: React with Human Hepatitis C Virus core protein. No reaction with HCV non-structural protein. Western Blot: Use of anti-HCV Core Protein antibody at 0.1 μg/mL will allow visualization of0.1 μg/lane of recombinant Capsid Protein C (CPC)+Envelope Protein M (EPM) (core), 0.5 μg/lane synthetic CPC, and 0.1 μg/lane recombinant chimeric HCV polyprotein. Testing is under both reducing and non-reducing conditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore with Double distillated water to adjust the final concentration to 1.0 mg/mL

Buffer: 0.01 M PBS, pH 7.0 without preservatives.

Preservative: Without preservative

Storage: -20 °C

Storage Comment: Store the antibody at -20 °C. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for HCV Core Protein

Target: HCV Core Protein (HCV C) (Hepatitis C Virus Core Protein (HCV C))

Alternative Name: HCV Core Protein (HCV C Products)

Target Type: Viral Protein

Background: The hepatitis C virus (HCV) core protein represents the first 191 amino acids of the viral precursor polyprotein and is cotranslationally inserted into the membrane of the endoplasmic reticulum. Hepatitis C virus (HCV) core is a viral structural protein, it also participates in some cellular processes, including transcriptional regulation. However the mechanisms of core-mediated transcriptional regulation remain poorly understood. Hepatitis C virus (HCV) core protein is thought to contribute to HCV pathogenesis through its interaction with various signal transduction pathways. In addition, HCV core antigen is a recently developed marker of hepatitis C infection. The HCV core protein has been previously shown to circulate in the bloodstream of HCV-infected patients and inhibit host immunity through an interaction with gC1qR. Hepatitis C Virus is a positive, single stranded RNA virus in the Flaviviridae family. The genome is approximately 10,000 nucleotides and encodes a single polyprotein of about 3,000 amino acids. The polyprotein is processed by host cell and viral proteases into three major structural proteins and several non structural proteins necessary for viral replication. Hepatitis C virus (HCV) causes most cases of non-A, non-B hepatitis and results in most HCV infected people developing chronic infections, liver cirrhosis and hepatocellular carcinoma. T cell responses, including interferon-gamma production are severely suppressed in chronic HCV patients.Synonyms: Hepatitis C Virus core protein