NDUFS3 antibody (Internal Region)

Catalog No. ABIN452425

Product Details anti-NDUFS3 Antibody

Target: NDUFS3 (NADH Dehydrogenase (Ubiquinone) Fe-S Protein 3, 30kDa (NADH-Coenzyme Q Reductase) (NDUFS3))

Binding Specificity: Internal Region

Reactivity: Human, Cow

Host: Goat

Clonality: Polyclonal

Conjugate: This NDUFS3 antibody is un-conjugated

Application: Western Blotting (WB)

Specificity: This antibody reacts to Complex I subunit NDUFS3.

Cross-Reactivity (Details): Species reactivity (expected):Bovine.
Species reactivity (tested):Human.

Purification: Affinity chromatography

Immunogen: Peptide with sequence C-DTRPTVRPRNDVAHK, from the internal region of the protein sequence

Application Details

Application Notes: Peptide ELISA: 1/4000. Western blot: 0.3 - 1 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0,5 mg/mL

Buffer: Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

Target Details for NDUFS3

Target: NDUFS3 (NADH Dehydrogenase (Ubiquinone) Fe-S Protein 3, 30kDa (NADH-Coenzyme Q Reductase) (NDUFS3))

Abstract: NDUFS3 Products

Background: The multisubunit NADH:ubiquinone oxidoreductase is the first enzyme complex in the electron transport chain of mitochondria. By use of chaotropic agents, complex I can be fragmented into 3 different fractions: a flavoprotein fraction, an iron-sulfur protein (IP) fraction, and a hydrophobic protein (HP) fraction. The IP fraction contains NDUFS1, NDUFS2, NDUFS3, NDUFS4, NDUFS5, NDUFS6, and NDUFA5.Synonyms: Complex I-30kD, Mitochondria Complex I (NADH Dehydrogenase), NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, NADH-ubiquinone oxidoreductase 30 kDa subunit, mitochondrial

Gene ID: 4722

NCBI Accession: NP_004542

UniProt: O75489

Pathways: Negative Regulation of intrinsic apoptotic Signaling