L-Selectin antibody (FITC)

Catalog No. ABIN452282

The Mouse Monoclonal anti-L-Selectin antibody has been validated for FACS. It is suitable to detect L-Selectin in samples from Human.

Quick Overview for L-Selectin antibody (FITC) (ABIN452282)

Target: L-Selectin (SELL) (Selectin L (SELL))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This L-Selectin antibody is conjugated to FITC

Application: Flow Cytometry (FACS)

Clone: DREG56

Product Details anti-L-Selectin Antibody (FITC)

Specificity: This antibody detects CD62L / L-selectin, a 65-76 kDa cell surface protein, expressed by neutrophils, monocytes, and subsets of T, B, and NK cells, that interacts with specific carbohydrates exposed on activated endothelial cells.

Cross-Reactivity (Details): Species reactivity (tested):Human

Characteristics: Synonyms: SELL, LNHR, LYAM1, Leu-8, TQ1, gp90-MEL, LECAM1, LAM-1, Lymph node homing receptor,Leukocyte adhesion molecule 1, Leukocyte surface antigen Leu-8, Leukocyte-endothelialcell adhesion molecule 1, CD62 antigen-like family member L

Immunogen: PMA-activated human peripheral blood leukocytes

Isotype: IgG1

Application Details

Application Notes: Flow cytometry analysis of human blood cells: 20 μL reagent / 100 μL of whole blood or10^6 cells in a suspension. The content of a vial (2 ml) is sufficient for 100 tests.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Buffer: Phosphate buffered saline (PBS) containing 15 mM sodium azide and 0.2 % (w/v) high-grade protease free Bovine Serum Albumin (BSA) as a stabilizing agent

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store the antibody undiluted at 2 - 8 °C. DO NOT FREEZE! This antibody is photosensitive and should be protected from light.
Shelf life: One year from despatch.

Expiry Date: 12 months

Target Details for L-Selectin

Target: L-Selectin (SELL) (Selectin L (SELL))

Alternative Name: CD62L / L-Selectin

Target Type: Chemical

Background: CD62L (L-selectin) is an adhesion glycoprotein that is constitutively expressed on the cell surface of leukocytes and mediates their homing to inflammatory sites and peripheral lymph nodes by enabling rolling along the venular wall. CD62L is also involved in activation-induced neutrophil aggregation. Activation-dependent CD62L shedding, however, counteracts neutrophil rolling. CD62L has also signaling roles including enhance of chemokine receptor expression. Similarly to CD62P, the major ligand of CD62L is PSGL-1 (P-selectin glycoprotein ligand-1). The level of CD62L expression can be used to help distinguish naive T cells from effector/memory T cells.Synonyms: CD62 antigen-like family member L, LAM-1, LECAM1, LNHR, LYAM1, Leu-8, Leukocyte adhesion molecule 1, Leukocyte surface antigen Leu-8, Leukocyte-endothelial cell adhesion molecule 1, Lymph node homing receptor, SELL, TQ1, gp90-MEL

Gene ID: 6402

UniProt: P14151