DLD antibody (Middle Region)

Catalog No. ABIN406692

Product Details anti-DLD Antibody

Target: DLD (Dihydrolipoamide Dehydrogenase (DLD))

Binding Specificity: Middle Region

Reactivity: Human, Mouse, Rat, Dog, Cow, Horse, Rabbit, Guinea Pig, Saccharomyces cerevisiae, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DLD antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Sequence: AGEMVNEAAL ALEYGASCED IARVCHAHPT LSEAFREANL AASFGKSINF

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 93%, Horse: 100%, Human: 100%, Mouse: 86%, Rabbit: 100%, Rat: 93%, Yeast: 86%, Zebrafish: 93%

Characteristics: This is a rabbit polyclonal antibody against DLD. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human DLD

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 509 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-DLD antibody (ABIN406692)

Ayanniyi, Raji, Adegoke: "Prevalence of asymptomatic sacroiliac joint dysfunction and its association with leg length discrepancies in male students in selected junior secondary schools in Ibadan." in: African journal of medicine and medical sciences, Vol. 37, Issue 1, pp. 37-42, (2008) (PubMed).

Target Details for DLD

Target: DLD (Dihydrolipoamide Dehydrogenase (DLD))

Alternative Name: DLD (DLD Products)

Background: DLD is the L protein of the mitochondrial glycine cleavage system. The L protein, also named dihydrolipoamide dehydrogenase, is also a component of the pyruvate dehydrogenase complex, the alpha-ketoglutarate dehydrogenase complex, and the branched-chain alpha-keto acide dehydrogenase complex. Mutations in this gene have been identified in patients with E3-deficient maple syrup urine disease and lipoamide dehydrogenase deficiency.This gene encodes the L protein of the mitochondrial glycine cleavage system. The L protein, also named dihydrolipoamide dehydrogenase, is also a component of the pyruvate dehydrogenase complex, the alpha-ketoglutarate dehydrogenase complex, and the branched-chain alpha-keto acide dehydrogenase complex. Mutations in this gene have been identified in patients with E3-deficient maple syrup urine disease and lipoamide dehydrogenase deficiency. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.
Alias Symbols: DLDH, E3, GCSL, LAD, PHE3
Protein Interaction Partner: HUWE1, FUS, UBC, SUMO1, NEDD8, MDM2, ASB15, PEPD, LDHA, CKB, ASS1, CNDP2, TNFAIP8, DPP3, RPL23, FN1, ATF2, MRPL11, UBAP2, MRPL37, MRPL13, MRPL42, GGH, UQCRC2, UQCRC1, MRPL23, NONO, NDUFB5, NDUFA9, ETFB, DLAT, DBT, AK2, SIRT7, SLC2A4, PSMD4, OGDH, DLD, PDH
Protein Size: 509

Molecular Weight: 56 kDa

Gene ID: 1738

NCBI Accession: NM_000108, NP_000099

UniProt: B2R5X0

Pathways: Ribonucleoside Biosynthetic Process, Cell RedoxHomeostasis