EIF5 antibody (N-Term)

Catalog No. ABIN406354

Product Details anti-EIF5 Antibody

Target: EIF5 (Eukaryotic Translation Initiation Factor 5 (EIF5))

Binding Specificity: N-Term

Reactivity: Human, Mouse, Rat, Cow, Horse, Dog, Guinea Pig, Rabbit, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EIF5 antibody is un-conjugated

Application: Western Blotting (WB)

Sequence: SVNVNRSVSD QFYRYKMPRL IAKVEGKGNG IKTVIVNMVD VAKALNRPPT

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 100%

Characteristics: This is a rabbit polyclonal antibody against EIF5. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human EIF5

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 431 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for EIF5

Target: EIF5 (Eukaryotic Translation Initiation Factor 5 (EIF5))

Alternative Name: EIF5 (EIF5 Products)

Background: EIF5 catalyzes the hydrolysis of GTP bound to the 40S ribosomal initiation complex (40S.mRNA.Met-tRNA[F].eIF-2.GTP) with the subsequent joining of a 60S ribosomal subunit resulting in the release of eIF-2 and the guanine nucleotide. The subsequent joining of a 60S ribosomal subunit results in the formation of a functional 80S initiation complex (80S.mRNA.Met-tRNA[F]).
Alias Symbols: EIF-5A
Protein Interaction Partner: DUSP12, HUWE1, UBC, TUBB, UBFD1, SCLY, ISOC1, MAT2B, FERMT2, YWHAQ, STK24, YWHAZ, YWHAH, YWHAE, EIF4H, VCL, SNRPD1, PCYT2, PFAS, PDHB, PAFAH1B1, HSPA9, HNRNPK, GNAI3, GLA, G6PD, DFFA, TBCB, CAPNS1, ALDH7A1, LMNA, PUM1, NRGN, FN1, EIF3B, RPL29, APP, Nedd4,
Protein Size: 431

Molecular Weight: 49 kDa

Gene ID: 1983

NCBI Accession: NM_183004, NP_892116

UniProt: P55010