NP (Middle Region) antibody

Catalog No. ABIN405995

Product Details

Target: NP

Binding Specificity: Middle Region

Reactivity: Human, Mouse, Rat, Cow, Rabbit, Saccharomyces cerevisiae, Dog, Zebrafish (Danio rerio), Guinea Pig, Horse

Host: Rabbit

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: Western Blotting (WB)

Sequence: GVDTLVVTNA AGGLNPKFEV GDIMLIRDHI NLPGFSGQNP LRGPNDERFG

Predicted Reactivity: Cow: 86%, Dog: 86%, Guinea Pig: 93%, Horse: 93%, Human: 100%, Mouse: 93%, Rabbit: 86%, Rat: 93%, Yeast: 91%, Zebrafish: 79%

Characteristics: This is a rabbit polyclonal antibody against NP. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human NP

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 289 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References

Ghanem, Li, Wing, Schramm: "Altered thermodynamics from remote mutations altering human toward bovine purine nucleoside phosphorylase." in: Biochemistry, Vol. 47, Issue 8, pp. 2559-64, (2008) (PubMed).

Target Details

Target: NP

Target Type: Viral Protein

Background: Defects in NP are the cause of nucleoside phosphorylase deficiency (NP deficiency). It leads to a severe T-cell immunodeficiency with neurologic disorder in children. The specific function of NP is not yet known.This gene encodes an enzyme which reversibly catalyzes the phosphorolysis of purine nucleosides. The enzyme is trimeric, containing three identical subunits. Mutations which result in nucleoside phosphorylase deficiency result in defective T-cell (cell-mediated) immunity but can also affect B-cell immunity and antibody responses. Neurologic disorders may also be apparent in patients with immune defects. A known polymorphism at aa position 51 that does not affect enzyme activity has been described. A pseudogene has been identified on chromosome 2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.
Alias Symbols: MGC117396, MGC125915, MGC125916, PNP, PRO1837, PUNP, NP
Protein Interaction Partner: PNP, UBC, SUMO2, PPME1, SBDS, PSAT1, GMPS, RAB1A, PSMD5, MTAP, ENO2, HECW2, ITGA4, NADK2, TMEM177, DNAJB11, NDUFV1, GLO1, UBD, CDK2, SIRT7, UCHL5, LRIF1, CCDC90B, RBM48, ZHX1, TFB2M, NCALD, TFAM, TP53, APLP1,
Protein Size: 289

Molecular Weight: 32 kDa

Gene ID: 4860

NCBI Accession: NM_000270, NP_000261

UniProt: P00491