RFC4 antibody (Middle Region)

Catalog No. ABIN405791

Product Details anti-RFC4 Antibody

Target: RFC4 (Replication Factor C (Activator 1) 4, 37kDa (RFC4))

Binding Specificity: Middle Region

Reactivity: Human, Rat, Mouse, Dog, Pig, Cow, Horse, Guinea Pig, Rabbit

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RFC4 antibody is un-conjugated

Application: Western Blotting (WB)

Sequence: DKLEAVVKDL IDEGHAATQL VNQLHDVVVE NNLSDKQKSI ITEKLAEVDK

Predicted Reactivity: Cow: 93%, Dog: 86%, Guinea Pig: 86%, Horse: 100%, Human: 100%, Mouse: 100%, Pig: 93%, Rabbit: 93%, Rat: 100%

Characteristics: This is a rabbit polyclonal antibody against RFC4. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human RFC4

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 363 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for RFC4

Target: RFC4 (Replication Factor C (Activator 1) 4, 37kDa (RFC4))

Alternative Name: RFC4 (RFC4 Products)

Background: The elongation of primed DNA templates by DNA polymerase delta and epsilon requires the action of the accessory proteins proliferating cell nuclear antigen (PCNA) and activator 1. This subunit may be involved in the elongation of the multiprimed DNA template.The elongation of primed DNA templates by DNA polymerase delta and DNA polymerase epsilon requires the accessory proteins proliferating cell nuclear antigen (PCNA) and replication factor C (RFC). RFC, also named activator 1, is a protein complex consisting of five distinct subunits of 140, 40, 38, 37, and 36 kD. This gene encodes the 37 kD subunit. This subunit forms a core complex with the 36 and 40 kDa subunits. The core complex possesses DNA-dependent ATPase activity, which was found to be stimulated by PCNA in an in vitro system. Alternatively spliced transcript variants encoding the same protein have been reported.
Alias Symbols: A1, MGC27291, RFC37
Protein Interaction Partner: RNF41, UBC, RFC5, PCNA, TP53, SUMO2, RPA3, RPA2, RPA1, RNF2, C14orf166, RTCB, RPL26L1, DIMT1, NELFB, EDC4, IGF2BP3, EIF2B2, EIF2B3, YBX3, QARS, NMT1, HNRNPM, MRE11A, ILF2, HNRNPU, FLII, FKBP3, EPRS, DHX9, DDX1, ABCF1, PARK2, TARDBP, MPG, RFC2, MYC, FN1, P
Protein Size: 363

Molecular Weight: 40 kDa

Gene ID: 5984

NCBI Accession: NM_002916, NP_002907

UniProt: P35249

Pathways: Telomere Maintenance, DNA Damage Repair, DNA Replication, Synthesis of DNA