NR0B2 antibody (Middle Region)

Catalog No. ABIN405753

Product Details anti-NR0B2 Antibody

Target: NR0B2 (Nuclear Receptor Subfamily 0, Group B, Member 2 (NR0B2))

Binding Specificity: Middle Region

Reactivity: Human, Rat, Rabbit, Dog, Horse, Guinea Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NR0B2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Sequence: AEAPVPSILK KILLEEPSSS GGSGQLPDRP QPSLAAVQWL QCCLESFWSL

Predicted Reactivity: Dog: 100%, Guinea Pig: 86%, Horse: 92%, Human: 100%, Rabbit: 100%, Rat: 79%

Characteristics: This is a rabbit polyclonal antibody against NR0B2. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human NR0B2

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 257 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for NR0B2

Target: NR0B2 (Nuclear Receptor Subfamily 0, Group B, Member 2 (NR0B2))

Alternative Name: NR0B2 (NR0B2 Products)

Background: NR0B2 is an unusual orphan receptor that contains a putative ligand-binding domain but lacks a conventional DNA-binding domain. It is a member of the nuclear hormone receptor family, a group of transcription factors regulated by small hydrophobic hormones, a subset of which do not have known ligands and are referred to as orphan nuclear receptors. The protein has been shown to interact with retinoid and thyroid hormone receptors, inhibiting their ligand-dependent transcriptional activation. In addition, interaction with estrogen receptors has been demonstrated, leading to inhibition of function. Studies suggest that the protein represses nuclear hormone receptor-mediated transactivation via two separate steps: competition with coactivators and the direct effects of its transcriptional repressor function.The protein encoded by this gene is an unusual orphan receptor that contains a putative ligand-binding domain but lacks a conventional DNA-binding domain. The gene product is a member of the nuclear hormone receptor family, a group of transcription factors regulated by small hydrophobic hormones, a subset of which do not have known ligands and are referred to as orphan nuclear receptors. The protein has been shown to interact with retinoid and thyroid hormone receptors, inhibiting their ligand-dependent transcriptional activation. In addition, interaction with estrogen receptors has been demonstrated, leading to inhibition of function. Studies suggest that the protein represses nuclear hormone receptor-mediated transactivation via two separate steps: competition with coactivators and the direct effects of its transcriptional repressor function. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.
Alias Symbols: SHP, SHP1
Protein Interaction Partner: TP53, SMAD4, IL3RA, HNRNPA1, FN1, PEF1, RBP5, ZMYND10, CHRD, ZAP70, RXRA, PLSCR1, Nr1i3, EID1, NR5A2, ESRRG, MDM2, RNF31, UBC, HDAC3, HDAC1, GPS2, SIN3A, EHMT2, TRAF6, SMARCA2, SNW1, VDR, RARG, PPARD, HNF4G, HNF4A, ESRRA, RUNX2, Prmt5, SIRT6, SIRT7, SIRT1
Protein Size: 257

Molecular Weight: 28 kDa

Gene ID: 8431

NCBI Accession: NM_021969, NP_068804

UniProt: Q15466

Pathways: Nuclear Receptor Transcription Pathway, Positive Regulation of Peptide Hormone Secretion, Intracellular Steroid Hormone Receptor Signaling Pathway, Steroid Hormone Mediated Signaling Pathway