PAPOLG antibody (Middle Region)

Catalog No. ABIN405370

Product Details anti-PAPOLG Antibody

Target: PAPOLG (Poly(A) Polymerase gamma (PAPOLG))

Binding Specificity: Middle Region

Reactivity: Human, Mouse, Rat, Guinea Pig, Cow, Dog, Horse, Rabbit, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PAPOLG antibody is un-conjugated

Application: Western Blotting (WB)

Sequence: SVDAIGGESM PIPTIDTSRK KRLPSKELPD SSSPVPANNI RVIKNSIRLT

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 79%

Characteristics: This is a rabbit polyclonal antibody against PAPOLG. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human PAPOLG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 736 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for PAPOLG

Target: PAPOLG (Poly(A) Polymerase gamma (PAPOLG))

Alternative Name: PAPOLG (PAPOLG Products)

Background: PAPOLG is a member of the poly(A) polymerase family which catalyzes template-independent extension of the 3' end of a DNA/RNA strand. This enzyme shares 60 % identity to the well characterized poly(A) polymerase II (PAPII) at the amino acid level. These two enzymes have similar organization of structural and functional domains. This enzyme is exclusively localized in the nucleus and exhibits both nonspecific and CPSF (cleavage and polyadenylation specificity factor)/AAUAAA-dependent polyadenylation activity.This gene encodes a member of the poly(A) polymerase family which catalyzes template-independent extension of the 3' end of a DNA/RNA strand. This enzyme shares 60 % identity to the well characterized poly(A) polymerase II (PAPII) at the amino acid level. These two enzymes have similar organization of structural and functional domains. This enzyme is exclusively localized in the nucleus and exhibits both nonspecific and CPSF (cleavage and polyadenylation specificity factor)/AAUAAA-dependent polyadenylation activity. This gene is located on chromosome 2 in contrast to the PAPII gene, which is located on chromosome 14.
Alias Symbols: FLJ11805, FLJ12972, FLJ13482, FLJ14187, MGC133307, MGC133308
Protein Interaction Partner: UBC, NPM1, DHX29, LZTR1, PCBD1, NFYA,
Protein Size: 736

Molecular Weight: 83 kDa

Gene ID: 64895

NCBI Accession: NM_022894, NP_075045

UniProt: Q9BWT3