KDM6A antibody (Middle Region)

Catalog No. ABIN405243

Product Details anti-KDM6A Antibody

Target: KDM6A (Lysine (K)-Specific Demethylase 6A (KDM6A))

Binding Specificity: Middle Region

Reactivity: Human, Mouse, Rat, Dog, Cow, Zebrafish (Danio rerio), Guinea Pig, Horse, Rabbit

Host: Rabbit

Clonality: Polyclonal

Conjugate: This KDM6A antibody is un-conjugated

Application: Western Blotting (WB)

Sequence: KTYIVHCQDC ARKTSGNLEN FVVLEQYKME DLMQVYDQFT LAPPLPSASS

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 86%

Characteristics: This is a rabbit polyclonal antibody against UTX. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human UTX

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 1401 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for KDM6A

Target: KDM6A (Lysine (K)-Specific Demethylase 6A (KDM6A))

Alternative Name: UTX (KDM6A Products)

Background: UTX is a histone demethylase that specifically demethylates 'Lys-27' of histone H3, thereby playing a central role in histone code. It demethylates trimethylated and dimethylated but not monomethylated H3 'Lys-27'. UTX plays a central role in regulation of posterior development, by regulating HOX gene expression. Demethylation of 'Lys-27' of histone H3 is concomitent with methylation of 'Lys-4' of histone H3, and regulates the recruitment of the PRC1 complex and monoubiquitination of histone H2A.
Alias Symbols: DKFZp686A03225, MGC141941, bA386N14.2, UTX, KABUK2
Protein Interaction Partner: KMT2D, WDR5, ASH2L, RBBP5, SMAD9, HIST2H3C, SRF, SMARCA4, GATA4, NKX2-5, TBX5, UBC, HIST1H3A, WDR82, PAXIP1, NCOA6, TLE1, PROSER1, KMT2C, N4BP2, PPP6R3, ZNF281, HIST3H3,
Protein Size: 1401

Molecular Weight: 154 kDa

Gene ID: 7403

NCBI Accession: NM_021140, NP_066963

UniProt: O15550

Pathways: Tube Formation, Warburg Effect