LIN28A antibody (N-Term)

Catalog No. ABIN404945

Product Details anti-LIN28A Antibody

Target: LIN28A (Lin-28 Homolog A (C. Elegans) (LIN28A))

Binding Specificity: N-Term

Reactivity: Human, Mouse, Rat, Cow, Dog, Pig, Sheep, Goat, Guinea Pig, Rabbit, Saccharomyces cerevisiae

Host: Rabbit

Clonality: Polyclonal

Conjugate: This LIN28A antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Sequence: GSVSNQQFAG GCAKAAEEAP EEAPEDAARA ADEPQLLHGA GICKWFNVRM

Predicted Reactivity: Cow: 100%, Dog: 100%, Goat: 91%, Guinea Pig: 86%, Human: 100%, Mouse: 90%, Pig: 100%, Rabbit: 100%, Rat: 100%, Sheep: 100%, Yeast: 77%

Characteristics: This is a rabbit polyclonal antibody against LIN28. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human LIN28

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 209 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for LIN28A

Target: LIN28A (Lin-28 Homolog A (C. Elegans) (LIN28A))

Alternative Name: LIN28 (LIN28A Products)

Background: LIN28 acts as a 'translational enhancer', driving specific mRNAs to polysomes and thus increasing the efficiency of protein synthesis. The association of LIN28 with the translational machinery and target mRNAs results in an increased number of initiation events per molecule of mRNA and, indirectly, in stabilizing the mRNAs. IT binds IGF2 mRNA, MYOD1 mRNA, ARBP/36B4 ribosomal protein mRNA and its own mRNA. LIN28 is Essential for skeletal muscle differentiation program through the translational up-regulation of IGF2 expression.
Alias Symbols: CSDD1, FLJ12457, LIN-28, LIN28A, ZCCHC1, LIN28, lin-28A
Protein Interaction Partner: HNRNPUL2, HNRNPA3, DHX36, SREK1, TRIM71, LARP4, TGS1, DHX57, GADD45GIP1, LTV1, NOA1, DDX50, KRI1, NOL6, METTL17, NOC3L, ZC3HAV1, PPAN, CCBL2, MEPCE, TSR1, NHP2, NOP10, STRBP, BRIX1, RBM28, PHAX, SRRT, LARP7, DDX41, HP1BP3, NOB1, PRPF19, ZBTB11, SND1, AATF
Protein Size: 209

Molecular Weight: 23 kDa

Gene ID: 79727

NCBI Accession: NM_024674, NP_078950

UniProt: Q9H9Z2

Pathways: Stem Cell Maintenance