DPF3 antibody (Middle Region)

Catalog No. ABIN404931

Product Details anti-DPF3 Antibody

Target: DPF3 (D4, Zinc and Double PHD Fingers, Family 3 (DPF3))

Binding Specificity: Middle Region

Reactivity: Human, Mouse, Rat, Guinea Pig, Horse, Rabbit, Cow, Dog

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DPF3 antibody is un-conjugated

Application: Western Blotting (WB)

Sequence: KDGFTSESTT LEALLRGEGV EKKVDAREEE SIQEIQRVLE NDENVEEGNE

Predicted Reactivity: Cow: 93%, Dog: 93%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%

Characteristics: This is a rabbit polyclonal antibody against DPF3. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human DPF3

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 357 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-DPF3 antibody (ABIN404931)

Theodorou, Speletas, Mamara, Papachristopoulou, Lazou, Scorilas, Katsantoni: "Identification of a STAT5 target gene, Dpf3, provides novel insights in chronic lymphocytic leukemia." in: PLoS ONE, Vol. 8, Issue 10, pp. e76155, (2013) (PubMed).

Hoyal, Kammerer, Roth, Reneland, Marnellos, Kiechle, Schwarz-Boeger, Griffiths, Ebner, Rehbock, Nelson, Braun: "Genetic polymorphisms in DPF3 associated with risk of breast cancer and lymph node metastases." in: Journal of carcinogenesis, Vol. 4, pp. 13, (2005) (PubMed).

Target Details for DPF3

Target: DPF3 (D4, Zinc and Double PHD Fingers, Family 3 (DPF3))

Alternative Name: DPF3 (DPF3 Products)

Background: DPF3 is a muscle-specific component of the BAF complex, a multiprotein complex involved in transcriptional activation and repression of select genes by chromatin remodeling (alteration of DNA-nucleosome topology). DPF3 specifically binds acetylated lysines on histone 3 and 4 (H3K14ac, H3K9ac, H4K5ac, H4K8ac, H4K12ac, H4K16ac). In the complex, it acts as a tissue-specific anchor between histone acetylations and methylations and chromatin remodeling. It thereby probably plays an essential role in heart and skeletal muscle development.
Alias Symbols: CERD4, FLJ14079
Protein Interaction Partner: SMARCA4, SMARCA2, RELA, NFKB1, SMARCD1, SMARCC1, SMARCB1, ESRRA,
Protein Size: 357

Molecular Weight: 40 kDa

Gene ID: 8110

NCBI Accession: NM_012074, NP_036206

UniProt: Q92784