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Myc Tag antibody (HRP)

WB, IHC (fro), EIA Host: Rabbit Polyclonal HRP

Catalog No. ABIN401754

Datasheet as PDF

Target See all Myc Tag products

Myc Tag

Reactivity

Please inquire
Host
76

49

6

5

5

5

1

1
Rabbit
Compatible Secondaries
Clonality
78

58

10
Polyclonal
Conjugate
68

13

10

7

3

3

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
This Myc Tag antibody is conjugated to HRP
Application
111

54

43

37

26

24

20

18

15

11

11

6

6

5

4

4

4

3

2

2

2

1

1

1

1

1
Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA)

Sequence

EQKLISEEDL

Specificity

This antibody is directed against human c-myc.

Characteristics

Absorption / Emission: 770 nm / 794 nm
Molar Ratio: 1,8 moles DyLight(TM) 800 per mole of Rabbit IgG

Purification

Affinity chromatography

Immunogen

Purified from whole rabbit serum prepared by repeated immunizations with Myc epitope tag peptide E-Q-K-L-I-S-E-E-D-L conjugated to KLH using maleimide. The sequence corresponds to aa 410-419 of human c-Myc.
anti-Myc Tag antibody
WB, IP, ELISA, IF Host: Mouse Monoclonal unconjugated

anti-Myc Tag (C-Term) antibody
Reactivity: Human WB, IP, IHC (p), FACS Host: Mouse Monoclonal 9E10 unconjugated

anti-Myc Tag antibody
WB, IP, ELISA Host: Rabbit Polyclonal unconjugated

anti-Myc Tag (C-Term) antibody (FITC)
Reactivity: Human FACS Host: Mouse Monoclonal 9E10 FITC

anti-Myc Tag (C-Term) antibody (Biotin)
Reactivity: Human WB, IP, IHC (p), FACS Host: Mouse Monoclonal 9E10 Biotin

anti-Myc Tag antibody
WB, IP, ELISA, IF Host: Mouse Monoclonal 2G8D5 unconjugated

anti-Myc Tag (AA 408-439) antibody
WB, ELISA Host: Mouse Monoclonal 9E10- unconjugated

anti-Myc Tag antibody
WB, IP, IF Host: Mouse Monoclonal 3B8 unconjugated

anti-Myc Tag (AA 410-419) antibody
WB, IP, IF, IHC, ICC Host: Mouse Monoclonal Myc-A7 unconjugated

anti-Myc Tag (AA 410-419) antibody
WB Host: Goat Polyclonal unconjugated

Application Notes

ELISA: 1/2,000 - 1/10,000. Western Blot: 1/1,000 - 1/5,000. Immunohistochemistry: 1/1,000 - 1/5,000.

Restrictions

For Research Use only
Reconstitution

Restore with 0.1 mL of deionized water (or equivalent).

Concentration

1.0 mg/mL (by UV absorbance at 280 nm)

Buffer

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 10 mg/mL BSA as stabilizer and 0.01 % (w/v) Gentamicin Sulfate as perservative

Preservative

Gentamicin sulfate

Precaution of Use

Use of sodium azide as a preservative will substantially inhibit the enzyme activity of horseradish peroxidase.

Handling Advice

Avoid repeated freezing and thawing.

Storage

4 °C/-20 °C

Storage Comment

Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
Target

Myc Tag

Alternative Name

C-Myc Epitope Tag (Myc Tag Products)

Target Type

Tag

Background

Epitope tags are short peptide sequences that are easily recognized by tag-specific antibodies. Due to their small size, epitope tags do not affect the tagged protein's biochemical properties. Most often sequences encoding the epitope tag are included with target DNA at the time of cloning to produce fusion proteins containing the epitope tag sequence. This allows anti-epitope tag antibodies to serve as universal detection reagents for any tag containing protein produced by recombinant means. This means that anti-epitope tag antibodies are a useful alternative to generating specific antibodies to identify, immunoprecipitate or immunoaffinity purify a recombinant protein. The anti-epitope tag antibody is usually functional in a variety of antibody-dependent experimental procedures. Expression vectors producing epitope tag fusion proteins are available for a variety of host expression systems including bacteria, yeast, insect and mammalian cells.Synonyms: c-myc tag, myc tag, myc-tag

Gene ID

4609

NCBI Accession

NP_002458

UniProt

P01106