CD20 antibody (C-Term)

Catalog No. ABIN401667

Product Details anti-CD20 Antibody

Target: CD20 (MS4A1) (Membrane-Spanning 4-Domains, Subfamily A, Member 1 (MS4A1))

Binding Specificity: C-Term

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This CD20 antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Sequence: CQDQESSPIE NDSSP

Specificity: This antibody recognizes CD20/MS4A1.

Cross-Reactivity (Details): Species reactivity (tested):Human.

Purification: Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide.

Immunogen: Peptide with sequence from the C Terminus of the protein sequence according to NP_068769.2, NP_690605.1. Genename: MS4A1

Application Details

Application Notes: Peptide ELISA: 1/8000. Western Blot: 3-5 μg/mL at Overnight incubation. Approx 37 kDa band seen in Daudi celllysates. Immunohistochemistry on Paraffin Sections: 2-5 μg/mL. In paraffin embedded Human Spleen, this antibody shows strong membranous staining inthe the follicles formed by B-cells.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH ~7.3 containing 0.02 % Sodium Azide as preserative and 0.5 % BSA as stabilizer.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

References for anti-CD20 antibody (ABIN401667)

Vargas-Inchaustegui, Demers, Shaw, Kang, Ball, Tuero, Musich, Mohanram, Demberg, Karpova, Li, Robert-Guroff: "Vaccine Induction of Lymph Node-Resident Simian Immunodeficiency Virus Env-Specific T Follicular Helper Cells in Rhesus Macaques." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 196, Issue 4, pp. 1700-10, (2016) (PubMed).

Target Details for CD20

Target: CD20 (MS4A1) (Membrane-Spanning 4-Domains, Subfamily A, Member 1 (MS4A1))

Alternative Name: CD20 (MS4A1 Products)

Synonyms: B1 antibody, Bp35 antibody, CD20 antibody, CVID5 antibody, LEU-16 antibody, MS4A2 antibody, S7 antibody, AA960661 antibody, Cd20 antibody, Ly-44 antibody, Ms4a2 antibody, MS4A1 antibody, bp35 antibody, cd20 antibody, ms4a2 antibody, leu-16 antibody, ms4a4c antibody, cd20-like antibody, membrane spanning 4-domains A1 antibody, membrane-spanning 4-domains, subfamily A, member 1 antibody, B-lymphocyte antigen CD20 antibody, MS4A1 antibody, Ms4a1 antibody, ms4a1 antibody, LOC101694281 antibody

Background: CD20 is a non glycosylated protein with a molecular weight of 35 or 37 kDa depending on the degree of phosphorylation. Although not a member of the tetraspanin superfamily of cell surface receptors, it crosses the cell membrane four times. The CD20 antigen is present on human pre B lymphocytes and on B lymphocytes at all stages of maturation, except on plasma cells. Low level expression of the CD20 antigen has been detected on normal T lymphocytes. The CD20 Molecule is involved in regulation of B cell differentiation, presumably via its reported function as a Ca++ channel subunit.Synonyms: B-cell marker, B-lymphocyte antigen CD20, B-lymphocyte surface antigen B1, Bp35, Leu-16, MS4A1, Membrane-spanning 4-domains subfamily A member 1

Gene ID: 931

NCBI Accession: NP_068769

UniProt: P11836