BRCA1 antibody (Ser1524)

Catalog No. ABIN401621

Product Details anti-BRCA1 Antibody

Target: BRCA1 (Breast Cancer 1 (BRCA1))

Binding Specificity: Ser1524

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This BRCA1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF)

Specificity: BRCA1 Antibody detects endogenous levels of total BRCA1protein.

Purification: Affinity chromatography

Immunogen: Synthesized non-phosphopeptide derived from human BRCA1 around the phosphorylation site of serine 1524 (Y-P-SP-Q-E)

Application Details

Application Notes: Immunofluorescence: 1/100 - 1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1,0 mg/mL

Buffer: Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Target Details for BRCA1

Target: BRCA1 (Breast Cancer 1 (BRCA1))

Alternative Name: BRCA1 / RNF53 (BRCA1 Products)

Background: BRCA1 (breast and ovarian cancer susceptibility protein 1) is a nuclear phosphoprotein that plays a role in maintaining genomic stability and acts as a tumor suppressor. It combines with other tumor suppressors, DNA damage sensors, and signal transducers to form a large multi-subunit protein complex known as BASC (for BRCA1 associated genome surveillance complex). BRCA1 associates with RNA polymerase II, and through the C terminal domain, also interacts with histone deacetylase complex. This protein thus plays a role in transcription, DNA repair of double-stranded breaks, and recombination. Mutations in the BRCA1 gene are responsible for approximately 40 % of inherited breast cancers and more than 80 % of inherited breast and ovarian cancers. Alternative splicing plays a role in modulating the subcellular localization and physiological function of this gene. Many alternatively spliced transcript variants have been described for this gene but only some have had their full length natures identified.Synonyms: Breast cancer type 1 susceptibility protein, RING finger protein 53

Gene ID: 672

NCBI Accession: NP_009225

UniProt: P38398

Pathways: Cell Division Cycle, DNA Damage Repair, Intracellular Steroid Hormone Receptor Signaling Pathway, Positive Regulation of Response to DNA Damage Stimulus