TNKS2 antibody (AA 800-900)

Catalog No. ABIN401534

Product Details anti-TNKS2 Antibody

Target: TNKS2 (Tankyrase, TRF1-Interacting Ankyrin-Related ADP-Ribose Polymerase 2 (TNKS2))

Binding Specificity: AA 800-900

Reactivity: Human, Mouse, Dog

Host: Goat

Clonality: Polyclonal

Conjugate: This TNKS2 antibody is un-conjugated

Application: Western Blotting (WB)

Specificity: This antibody detects Tankyrase-2 (TANK2). No cross-reactivity expected with TANK1.

Cross-Reactivity (Details): Species reactivity (expected):Mouse and Canine (Dog).
Species reactivity (tested):Human.

Purification: Ammonium Sulphate Precipitation followed by Affinity Chromatography using the immunizing peptide.

Immunogen: Fusion protein containing 73 aa from aa 800-900 region of the protein sequence according to NP_079511.1. Genename: TNKS2

Application Details

Application Notes: Western blot: 0.5-1.5 μg/mL. In transfected HEK293 transiently expressing TANK2 a band of approx.127 kDa is observed. This band is not observed in the non-transfected HEK293. The calculated molecular size is127 kDa according to NP_079511.1. Full caption (see picture below): HEK293 overexpressing TANK2 (lane 4) and TANK1 (lane 2)and probed with ABIN401534 (mock transfection in first lane). Lane three is empty. Lowerpanel shows the same lysates probed for alpha-Actin to show protein levels. Primaryincubation (0.5 μg/mL) was overnight at 4 °C. Detected by chemiluminescence.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH 7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

Target Details for TNKS2

Target: TNKS2 (Tankyrase, TRF1-Interacting Ankyrin-Related ADP-Ribose Polymerase 2 (TNKS2))

Alternative Name: Tankyrase-2 (TANK2) (TNKS2 Products)

Synonyms: ARTD6 antibody, PARP-5b antibody, PARP-5c antibody, PARP5B antibody, PARP5C antibody, TANK2 antibody, TNKL antibody, pART6 antibody, 5430432P15Rik antibody, AA517131 antibody, AI662480 antibody, TNKS-2 antibody, Tank2 antibody, tankyrase-2 antibody, TNKS2 antibody, tankyrase-1 antibody, tnks antibody, tnks.L antibody, tnks2 antibody, tankyrase 2 antibody, tankyrase, TRF1-interacting ankyrin-related ADP-ribose polymerase 2 antibody, tankyrase, TRF1-interacting ankyrin-related ADP-ribose polymerase S homeolog antibody, TNKS2 antibody, Tnks2 antibody, tnks2 antibody, tnks.S antibody

Background: Tankyrase 2 may regulate vesicle trafficking and modulate the subcellular distribution of SLC2A4/GLUT4-vesicles. It has PARP activity and can modify TRF1, and thereby contribute to the regulation of telomere length. Tankyrase 2 is highly expressed in placenta, skeletal muscle, liver, brain, kidney, heart, thymus, spinal cord, lung, peripheral blood leukocytes, pancreas, lymph nodes, spleen, prostate, testis, ovary, small intestine, colon, mammary gland, breast and breast carcinoma, and in common-type meningioma. Also highly expressed in fetal liver, heart and brain. Tankyrase 2 shares approximately 83 % sequence identity with Tankyrase 1.Synonyms: PARP5B, TNKL, TNKS-2, TNKS2, TRF1-interacting ankyrin-related ADP-ribose polymerase 2, Tankyrase II, Tankyrase-like protein, Tankyrase-related protein

Gene ID: 80351

NCBI Accession: NP_079511

UniProt: Q9H2K2