SNX26 antibody (C-Term)

Catalog No. ABIN401527

Product Details anti-SNX26 Antibody

Target: SNX26 (Sorting Nexin 26 (SNX26))

Binding Specificity: C-Term

Reactivity: Human, Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: This SNX26 antibody is un-conjugated

Application: Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Sequence: SWSLHSEGQT RSYC

Specificity: This antibody detects SNX26 at C-term.

Cross-Reactivity (Details): Species reactivity (expected):MouseSpecies reactivity (tested):Human

Purification: Ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide

Immunogen: Peptide from the C Terminus of the protein sequence according to NP_443180.2. Genename: SNX26

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1: 8000. Western blot: Preliminary experiments gave an approx 75 kDa band in Human Bone Marrow,Kidney, and Pancreas lysates after 1 μg/mL antibody staining. ABIN401527 may detect theisoform A1A5D2 (UniProtKB) with a molecular weight of 81.5 kDa which is similar in aminoacid sequence to NP_443180.2 however lacking aa729-1268. Please note that currently wecannot find a precise explanation in the literature for the band we observe given thecalculated size of 125 kDa according to NP_443180.2. The 75 kDa band was successfullyblocked by incubation with the immunizing peptide. We would appreciate any feedbackfrom people in the field - have any results been reported with other antibodies/lysates?Have any further splice variants/modified forms been reported?Immunohistochemistry on paraffin sections: 2.5 μg/mL. In Human Brain shows cytoplasmstaining of Purkinje cells in Cerebellum.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C.

Target Details for SNX26

Target: SNX26 (Sorting Nexin 26 (SNX26))

Alternative Name: Sorting Nexin-26 (SNX26) (SNX26 Products)

Background: SNX26 may be involved in several stages of intracellular trafficking. It could play an important role in the regulation of glucose transport by insulin. It may act as a downstream effector of RHOQ/TC10 in the regulation of insulin-stimulated glucose transport.Synonyms: ARHGAP33, Rho GTPase-activating protein 33, TCGAP

Gene ID: 115703

NCBI Accession: NP_443180

UniProt: O14559