FITC antibody

Catalog No. ABIN401492

Product Details anti-FITC Antibody

Target: FITC (Fluorescein Isothiocyanate (FITC))

Reactivity: Please inquire

Host: Goat

Clonality: Polyclonal

Conjugate: This FITC antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Purification: Immunoaffinity chromatography using Fluorescein coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation

Immunogen: Fluorescein conjugated to Goat IgG

Isotype: IgG

Application Details

Application Notes: Suitable for highly specific immunological methods requiring extremely low backgroundlevels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity. ELISA: 1: 2,000 - 1: 8,000. Western blot: 1: 200 - 1: 1,000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL (by UV absorbance at 280 nm)

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing. Should this product contain a precipitate we recommend microcentrifugation before use.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer.

Target Details for FITC

Target: FITC (Fluorescein Isothiocyanate (FITC))

Alternative Name: FITC (FITC Products)

Target Type: Chemical

Background: Synonyms: Fluorescein Isothiocyanate