EIF3E antibody (C-Term)

Catalog No. ABIN401422

Product Details anti-EIF3E Antibody

Target: EIF3E (Eukaryotic Translation Initiation Factor 3 Subunit E (EIF3E))

Binding Specificity: C-Term

Reactivity: Human, Mouse, Rat, Cow, Dog, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EIF3E antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Specificity: This antibody is specific for Mouse eIF3S6/Int6 protein.

Cross-Reactivity (Details): Species reactivity (expected):Human, Rat, Canine, Bovine, Monkey.
Species reactivity (tested):Mouse.

Purification: Immunoaffinity Chromatography.

Immunogen: Synthetic peptide corresponding to a region near the C-terminus of mouse EIF3S6/Int6 . Genename: EIF3E

Isotype: IgG

Application Details

Application Notes: Suitable for use in ELISA (1/80,000) and Western Blot (1/1,000). Expect a band approx. 48 kDa in size corresponding to eIF3S6/Int6 by Western blotting in theappropriate cell lysate or extract. This antibody is capable of detecting bothover-expressed and endogenous eIF3S6/Int6.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.8 mg/mL (by UV absorbance at 280 nm)

Buffer: 0.02 M Potassium Phosphate, 0.12 M Sodium Chloride, pH 7.2 containing 0.01 % (w/v) Sodium Azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Target Details for EIF3E

Target: EIF3E (Eukaryotic Translation Initiation Factor 3 Subunit E (EIF3E))

Alternative Name: EIF3E / EIF3S6 (EIF3E Products)

Background: Int6 is a candidate tumor suppressor in multiple neoplasms, and in particular, breast and lung cancers. The Int6 locus was initially identified as a common insertion site (CIS) in a genetic screen for transforming sequences in a breast cancer mouse model system. Insertion of mouse mammary tumor virus (MMTV) into this locus results in the production of an amino-terminal truncated gene product. Expression of the truncated Int6 product corresponds to cellular transformation in both in vivo and in vitro systems. This gene product plays a role in regulating translation initiation and is a component of the eIF3 translation initiation complex. There is evidence that suggests that Int6 may impart a negative role in the general translational machinery while promoting an increase in the expression of a subset of stress-responsive genes. Taken together, it is of great interest to further study the mechanism by which Int6 is involved in regulating cell growth.Synonyms: Eukaryotic translation initiation factor 3 subunit 6, Eukaryotic translation initiation factor 3 subunit E, INT6, Viral integration site protein INT-6 homolog, eIF-3 p48

Gene ID: 16341

NCBI Accession: NP_032414

UniProt: P60229

Pathways: Ribonucleoprotein Complex Subunit Organization, Hepatitis C