SMAD4 antibody (C-Term)

Catalog No. ABIN401410

Product Details anti-SMAD4 Antibody

Target: SMAD4 (SMAD Family Member 4 (SMAD4))

Binding Specificity: C-Term

Reactivity: Human, Mouse, Xenopus laevis

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SMAD4 antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Specificity: This antibody is directed against SMAD4 protein.

Cross-Reactivity (Details): Species reactivity (expected):Mouse, Xenopus.
Species reactivity (tested):Human.

Purification: Affinity chromatography

Immunogen: Synthetic peptide corresponding to a region near the carboxy terminus of human SMAD4 protein

Isotype: IgG

Application Details

Application Notes: ELISA: 1/15,000 - 1/60,000. Western Blot: 1/500 - 1/2,000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.2 mg/mL (by UV absorbance at 280 nm)

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Target Details for SMAD4

Target: SMAD4 (SMAD Family Member 4 (SMAD4))

Alternative Name: SMAD4 (SMAD4 Products)

Background: SMAD4 (also known as Mothers against decapentaplegic homolog 4, Mothers against DPP homolog 4, deletion target in pancreatic carcinoma 4 and hSMAD4) is a common mediator of signal transduction by TGF-beta (transforming growth factor), but is also involved in cancer development and metastases as a tumor suppressor. SMAD4 promotes binding of the SMAD2/SMAD4/FAST-1 complex to DNA and provides an activation function required for SMAD1 or SMAD2 to stimulate transcription. SMAD4 may form trimers with receptor-regulated SMAD (R-SMAD) and interacts with ATF2, COPS5, DACH1, MSG1, SKI and TRIM33. In the absence of ligand SMAD4 is found in the cytoplasm, but when complexed with R-SMAD, translocates to the nucleus. Defects in SMAD4 are a cause of pancreatic carcinoma and juvenile polyposis syndrome (JPS), a syndrome in which patients are at risk for developing gastrointestinal cancers.Synonyms: DPC4, Deletion target in pancreatic carcinoma 4, MAD homolog 4, MADH4, Mothers against DPP homolog 4, Mothers against decapentaplegic homolog 4, SMAD 4, SMAD family member 4, SMAD-4

Gene ID: 4089

NCBI Accession: NP_005350

UniProt: Q13485

Pathways: Cell Division Cycle, Chromatin Binding, Autophagy