UBE2J1 antibody (N-Term)

Catalog No. ABIN401384

Product Details anti-UBE2J1 Antibody

Target: UBE2J1 (Ubiquitin-Conjugating Enzyme E2, J1, U (UBE2J1))

Binding Specificity: N-Term

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This UBE2J1 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Enzyme Immunoassay (EIA)

Specificity: This antibody is directed against Ube2j1 protein.

Cross-Reactivity (Details): Species reactivity (expected):Mouse, Rat.
Species reactivity (tested):Human.

Purification: Affinity chromatography

Immunogen: Synthetic peptide corresponding to residues near the amino terminus of the human Ube2j1 protein

Isotype: IgG

Application Details

Application Notes: ELISA: 1/1,000 - 1/5,000. Western Blot: 1/200 - 1/2,000. Immunoprecipitation: 1 - 2 μg.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.71 mg/mL (by UV absorbance at 280 nm)

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Target Details for UBE2J1

Target: UBE2J1 (Ubiquitin-Conjugating Enzyme E2, J1, U (UBE2J1))

Alternative Name: UBE2J1 (UBE2J1 Products)

Background: Ube2j1 and Ube2j2 are homologs of the yeast ubiquitin-conjugating enzyme UBC6, which catalyzes the covalent attachment of ubiquitin to other proteins. These proteins constitute a distinct family of ubiquitin-conjugating enzymes sharing a conserved non-canonical active site sequence and a C-terminal transmembrane domain. By analogy with yeast UBC6, Ube2j1 and Ube2j2 are localized to the endoplasmic reticulum and seem to function in the selective degradation of misfolded membrane proteins and in general mediation of the stress response.Synonyms: CGI-76, HSPC153, HSPC205, HSUBC6e, NCUBE1, Non-canonical ubiquitin-conjugating enzyme 1, Ubiquitin-conjugating enzyme E2 J1, Yeast ubiquitin-conjugating enzyme UBC6 homolog E

Gene ID: 51465

NCBI Accession: NP_057105

UniProt: Q9Y385