RNF25 antibody (C-Term)
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- Target See all RNF25 Antibodies
- RNF25 (Ring Finger Protein 25 (RNF25))
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Binding Specificity
- C-Term
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Reactivity
- Human, Mouse
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This RNF25 antibody is un-conjugated
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Application
- Western Blotting (WB), Immunoprecipitation (IP), Enzyme Immunoassay (EIA)
- Specificity
- This antibody is directed against RNF25 protein.
- Purification
- Affinity chromatography
- Immunogen
- Synthetic peptide corresponding to amino acids near the carboxyl terminus of human RNF25 protein
- Isotype
- IgG
- Top Product
- Discover our top product RNF25 Primary Antibody
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- Application Notes
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ELISA: 1/10,000 - 1/40,000. Western Blot: 1/200 - 1/2,000. Immunoprecipitation: 1 μg.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - Restrictions
- For Research Use only
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- Concentration
- 1.0 mg/mL (by UV absorbance at 280 nm)
- Buffer
- 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 0.01 % (w/v) Sodium Azide
- Preservative
- Sodium azide
- Precaution of Use
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Avoid repeated freezing and thawing.
- Storage
- -20 °C
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- Target
- RNF25 (Ring Finger Protein 25 (RNF25))
- Alternative Name
- RNF25 (RNF25 Products)
- Background
- RING finger protein 25 (RNF25, also named AO7) contains a RING finger domain and is ubiquitously expressed in various tissues. RNF25 was initially identified in a yeast two-hybrid screen of a murine T-cell library by using UbcH5b, an E2 enzyme, as bait. RNF25 has also been shown to act as a putative E3 ligase, at least in vitro. RNF25 localizes predominantly in the nucleus and supports the transcriptional activity of NF-kappaB by interacting with p65 in vivo upon stimulation with TNF. Yeast two-hybrid data also suggest that RNF25 interacts with a number of other molecules which may be potential ubiquitin ligase substrates. Among these are molecules that have critical roles in signal transduction and in regulation of translation (personal communication, Allan Weissman, CCR-NCI, Bethesda, MD).Synonyms: E3 ubiquitin-protein ligase RNF25, RING finger protein 25
- Gene ID
- 64320
- NCBI Accession
- NP_071898
- UniProt
- Q96BH1
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