TUBA1B antibody (AA 427-441)

Catalog No. ABIN401310

Product Details anti-TUBA1B Antibody

Target: TUBA1B (Tubulin, alpha 1B (TUBA1B))

Binding Specificity: AA 427-441

Reactivity: Human, Avian, Mammalian

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TUBA1B antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Specificity: This antibody is directed against alpha Tubulin protein. Cross reactivity will occur with all isoforms of alpha tubulin. Such broad reactivity makes this antibody useful as an excellent Loading Control.

Purification: Immunoaffinity Chromatography.

Immunogen: Synthetic peptide corresponding to amino acids 427-441 of Human alpha Tubulin

Isotype: IgG

Application Details

Application Notes: ELISA: 1/5,000-1/25,000. Immunofluorescence: 1/500-1/2,000Western Blot: 1/500-1/3,000. Expect a band at approx. 50 kDa in size corresponding to alpha tubulin by Western blotting inmost cell lysates or extracts.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.08 mg/mL (by UV absorbance at 280 nm)

Buffer: 0.02 M Potassium Phosphate, 0.12 M Sodium Chloride, pH 7.2 containing 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Target Details for TUBA1B

Target: TUBA1B (Tubulin, alpha 1B (TUBA1B))

Alternative Name: alpha Tubulin / TUBA1B (TUBA1B Products)

Background: Microtubules are involved in a wide variety of cellular activities ranging from mitosis and transport events to cell movement and the maintenance of cell shape. Tubulin itself is a globular protein consisting of two polypeptides (alpha and beta tubulin). Alpha and beta tubulin dimers are assembled to 13 protofilaments that form a microtubule of 22-nm diameter. Tyrosine ligase adds a C-terminal tyrosine to monomeric alpha tubulin. Assembled microtubules can again be detyrosinated by a cytoskeleton-associated carboxypeptidase. Detyrosinated alpha tubulin is referred to as Glu-tubulin. Another post-translational modification of detyrosinated alpha tubulin is C-terminal polyglutamylation, which is characteristic of microtubules in neuronal cells and the mitotic spindle.Synonyms: Alpha-tubulin ubiquitous, Tubulin K-alpha-1, Tubulin alpha-1B chain, Tubulin alpha-ubiquitous chain

Gene ID: 10376

NCBI Accession: NP_006073

UniProt: P68363

Pathways: Microtubule Dynamics, M Phase