Swi6 (AA 314-328) antibody

Catalog No. ABIN401300

Product Details

Target: Swi6

Binding Specificity: AA 314-328

Reactivity: Saccharomyces cerevisiae

Host: Rabbit

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Specificity: This antibody reacts to Swi6.

Purification: Affinity chromatography

Immunogen: Synthetic peptide corresponding aa 314-328 of S.pombe Swi6 protein

Isotype: IgG

Application Details

Application Notes: ELISA: 1/5,000 - 1/30,000. Western Blot: 1/500 - 1/3,000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.7 mg/mL (by UV absorbance at 280 nm)

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Target Details

Target: Swi6

Background: Swi6 is also known as chromatinassociated swi6 protein and repression of silent mating type loci protein. This protein recognizes and binds to histone H3 tails that are methylated at Lys-9, leading to epigenetic repression. Swi6 is also involved in the repression of the silent mating-type loci MAT2 and MAT3 and it may be resposible for the compaction of MAT2/3 into a heterochromatin-like conformation which represses the transcription of these silent cassettes. Swi6 also interacts with the cohesin subunit psc3. Swi6 has a nuclear localization and contains 2 chromo domains.Synonyms: Chromatin-associated protein swi6

Gene ID: 2541633

NCBI Accession: NP_593449

UniProt: P40381