SPRY4 antibody (AA 306-322)

Catalog No. ABIN401284

Product Details anti-SPRY4 Antibody

Target: SPRY4 (Sprouty RTK Signaling Antagonist 4 (SPRY4))

Binding Specificity: AA 306-322

Reactivity: Human, Mouse, Rat, Cow, Dog, Chimpanzee

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SPRY4 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Specificity: This affinity purified antibody is directed against Sprouty-4 protein.

Cross-Reactivity (Details): Species reactivity (expected):Chimpanzee (100 %).Bovine (94 %).Mouse (88 %).Rat and dog (82 %).
Species reactivity (tested):Human.

Purification: Immunoaffinity chromatography

Immunogen: Synthetic peptide corresponding to amino acids 306-322 of Human Sprouty-4 protein

Isotype: IgG

Application Details

Application Notes: ELISA: 1: 3,000 - 1: 12,000. Western blot: 1: 100 - 1: 1,000 (Expect a band (maybe a doublet) approx. 35 kDa in sizecorresponding to Sprouty-4 in the appropriate cell lysate or extract). Immunohistochemistry on paraffin sections: 1 μg/mL to 10 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.93 mg/mL (by UV absorbance at 280 nm)

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing. Should this product contain a precipitate we recommend microcentrifugation before use.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer.

Target Details for SPRY4

Target: SPRY4 (Sprouty RTK Signaling Antagonist 4 (SPRY4))

Alternative Name: Sprouty Homolog 4 / SPRY4 (SPRY4 Products)

Background: Sprouty-4 (also known as SPRY4) is an inhibitor of the insulin receptor and EGFR-transduced mitogen-activated protein kinase (MAPK) signaling pathway downstream of FGF and EGF receptor tyrosine kinase activation. It is positioned upstream of RAS activation and impairs the formation of active GTP-RAS. SPRY4 is widely expressed, with different isoforms. The protein consists of 322 amino acids, with a cysteine-rich region, Src homology 3 binding regions, proline-rich regions, and a PEST sequence. It is expressed predominantly in the cytoplasm. Northern results show bands across most tissues, with strongest expression in heart, brain, placenta, lung, and intestine.Synonyms: Sprouty 4, Spry-4

Gene ID: 81848

NCBI Accession: NP_001120968

UniProt: Q9C004