PNKP antibody (Internal Region)

Catalog No. ABIN401253

Product Details anti-PNKP Antibody

Target: PNKP (Polynucleotide Kinase 3'-Phosphatase (PNKP))

Binding Specificity: Internal Region

Reactivity: Human, Chimpanzee

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PNKP antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Specificity: This affinity purified antibody is directed against PNK protein.

Cross-Reactivity (Details): Species reactivity (expected):Chimpanzee (100 %).Dog, mouse and rat (83 %).
Species reactivity (tested):Human.

Purification: Immunoaffinity chromatography

Immunogen: Synthetic peptide corresponding to an internal region of human PNK protein

Isotype: IgG

Application Details

Application Notes: ELISA: 1: 3,000 - 1: 12,000. Western blot: 1: 300 - 1: 1,500 (Expect a band approximately 57 kDa in size corresponding toPNK in the appropriate cell lysate or extract).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.46 mg/mL (by UV absorbance at 280 nm)

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing. Should this product contain a precipitate we recommend microcentrifugation before use.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody at 2 - 8 °C up to one month or for longer (in aliquots) at -20 °C or below.

Target Details for PNKP

Target: PNKP (Polynucleotide Kinase 3'-Phosphatase (PNKP))

Alternative Name: PNKP (PNKP Products)

Background: Mammalian PNK (also known as PNKP, Polynucleotide kinase 3'-phosphatase, DNA 5'-kinase/3'- hosphatase and Polynucleotide kinase) catalyzes the 5' phosphorylation of nucleic acids and can have associated 3' phosphatase activity, predictive of an important function in DNA repair following ionizing radiation or oxidative damage. PNK is a nuclear protein that is expressed in many tissues with highest expression in spleen and testis, and lowest expression in small intestine.Synonyms: Bifunctional polynucleotide phosphatase/kinase, DNA 5'-kinase/3'-phosphatase, Polynucleotide kinase-3'-phosphatase

Gene ID: 11284

NCBI Accession: NP_009185

UniProt: Q96T60

Pathways: DNA Damage Repair, Nucleotide Phosphorylation