USP18 antibody (AA 40-49)

Catalog No. ABIN401252

Product Details anti-USP18 Antibody

Target: USP18 (Ubiquitin Specific Peptidase 18 (USP18))

Binding Specificity: AA 40-49

Reactivity: Human, Chimpanzee, Orang-Utan

Host: Rabbit

Clonality: Polyclonal

Conjugate: This USP18 antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Specificity: This antibody is directed against human UBP43 protein.

Cross-Reactivity (Details): Species reactivity (expected):A BLAST analysis was used to suggest reactivity with this protein from chimpanzee and orangutan based on 100 % homology for the immunogen sequence.
Species reactivity (tested):Human.

Purification: Immunoaffinity chromatography

Immunogen: Synthetic peptide corresponding aa 40-49 of human UBP43 protein

Isotype: IgG

Application Details

Application Notes: ELISA: 1: 10,000 - 1: 50,000. Western blot: 1: 500 - 1: 2,000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.9 mg/mL (by UV absorbance at 280 nm)

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing. Should this product contain a precipitate we recommend microcentrifugation before use.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody at 2 - 8 °C up to one month or for longer (in aliquots) at -20 °C or below.

Target Details for USP18

Target: USP18 (Ubiquitin Specific Peptidase 18 (USP18))

Alternative Name: USP18 (USP18 Products)

Background: UBP43 (also known as ISG43, Ubl thiolesterase 18, ISG15-specific processing protease, 43 kDa ISG15-specific protease, ubiquitin specific protease 18 or USP18) is a member of the deubiquitinating protease family of enzymes, which removes ubiquitin adducts from a broad range of protein substrates. Deletion of the UBP43 gene in mouse leads to a massive increase of ISG15 conjugates in tissues indicating that UBP43 is a major ISG15-specific protease. UBP43 is the first ISG15-specific protease reported. Both ISG15 and UBP43 genes are known to be strongly induced by interferon, genotoxic stress, and viral infection. Thus UBP43 may be necessary to maintain a critical cellular balance of ISG15-conjugated proteins in both healthy and stressed organisms.Synonyms: 43 kDa ISG15-specific protease, ISG15-specific-processing protease, ISG43, Ubl carboxyl-terminal hydrolase 18, Ubl thioesterase 18

Gene ID: 11274

NCBI Accession: NP_059110

UniProt: Q9UMW8