SFRP1 antibody

Catalog No. ABIN401251

Product Details anti-SFRP1 Antibody

Target: SFRP1 (Secreted Frizzled-Related Protein 1 (SFRP1))

Reactivity: Human, Mouse, Rat, Cow

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SFRP1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF), Enzyme Immunoassay (EIA)

Specificity: This affinity purified antibody is directed against Human SFRP protein. A BLAST analysis was used to suggest reactivity with this protein from Human based on 100 % homology for the immunogen sequence. Expect cross reactivity with SFRP1 from Mouse, Rat and Bovine sources as only a single amino acid residue change is found within the immunogen sequence (91 % positive by BLAST). Cross reactivity with SFRP1 homologues from other sources has not been determined.

Cross-Reactivity (Details): Species reactivity (expected):Mouse, Rat and Bovine.
Species reactivity (tested):Human.

Purification: Immunoaffinity Chromatography

Immunogen: Synthetic peptide corresponding to a 12 amino acid region of Human Sfrp1 protein

Isotype: IgG

Application Details

Application Notes: ELISA: 1: 5,000 - 1: 25,000. Immunohistochemistry on paraffin sections: 1: 800. Western blot: 1: 200 - 1: 2,000 (Expect a band approximately 37 kDa in size corresponding toSfrp1 in the appropriate cell lysate or extract).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.56 mg/mL (by UV absorbance at 280 nm)

Buffer: 0.02 M Potassium Phosphate, 0.12 M Sodium Chloride, pH 7.2, 0.01 % (w/v) Sodium Azide, None

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

Target Details for SFRP1

Target: SFRP1 (Secreted Frizzled-Related Protein 1 (SFRP1))

Alternative Name: SFRP1 (SFRP1 Products)

Background: SFRP1 (also known as FRP, FRP1, SARP2, Secreted Apoptosis-related Protein 2, Secreted Frizzledrelated Protein and Secreted frizzled-related protein 1) is a member of the SFRP family that contains a cysteine-rich domain homologous to the putative Wnt-binding site of Frizzled proteins. SFRPs act as soluble modulators of Wnt signaling. SFRP1 and SFRP5 may be involved in determining the polarity of photoreceptor cells in the retina. SFRP1 is expressed in several human tissues, with the highest levels in heart.Synonyms: FRP, FRP-1, FRP1, SARP-2, SARP2, SFRP-1, Secreted apoptosis-related protein 2, Secreted frizzled-related protein 1

Molecular Weight: 35.4 kDa (Predicted)

Gene ID: 6422

NCBI Accession: NP_003003

UniProt: Q8N474

Pathways: WNT Signaling, Intracellular Steroid Hormone Receptor Signaling Pathway, Negative Regulation of Hormone Secretion, Regulation of Intracellular Steroid Hormone Receptor Signaling, Stem Cell Maintenance, Tube Formation, Positive Regulation of fat Cell Differentiation