HA-Tag antibody

Catalog No. ABIN401240

Product Details anti-HA-Tag Antibody

Target: HA-Tag

Reactivity: Please inquire

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HA-Tag antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Sequence: YPYDVPDYA

Purification: Affinity Chromatography

Immunogen: Whole rabbit serum prepared by repeated immunizations with the 9-aa epitope tag peptide YPYDVPDYA (114-122) from Hemagglutinin Influenza conjugated to KLH using maleimide. A residue of cysteine was added to the carboxy terminal end to facilitate coupling.

Isotype: IgG

Application Details

Application Notes: Anti-HA is optimally suited for monitoring the expression of HA-tagged fusion proteins. As such, anti-HA/HA can be used to identify fusion proteins containing the HA epitope. Theantibody recognizes the HA epitope tag fused to the amino- or carboxy- termini of targetedproteins, as expressed in many commonly used expression vectors. This antibody has been tested by ELISA and Western blotting against both the immunizingpeptide and HA containing recombinant proteins. This antibody is likely functional forImmunoprecipitation, Immunocytochemistry, and other Immunodetection techniques. Affinity purification of the polyclonal antibody results in very low background levels inassays and low cross-reactivity with other cellular proteins. Recommended Dilutions: ELISA: 1/20,000-1/150,000.

Restrictions: For Research Use only

Handling

Concentration: 0.95 mg/mL (by UV absorbance at 280 nm)

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

Target Details for HA-Tag

Target: HA-Tag

Alternative Name: HA Epitope Tag (YPYDVPDYA) (HA-Tag Products)

Target Type: Tag

Background: Epitope tags are short peptide sequences that are easily recognized by tag-specific antibodies. Due to their small size, epitope tags do not affect the biochemical properties of the tagged protein. Most often, sequences encoding the epitope tag are included with the target DNA at the time of cloning to produce fusion proteins containing the epitope tag sequence. This allows anti-epitope tag antibodies to serve as universal detection reagents for any tag containing protein produced by recombinant means. This means that anti-epitope tag antibodies are a useful alternative to generating specific antibodies to identify, immunoprecipitate or immunoaffinity purify a recombinant protein. The anti-epitope tag antibody is usually functional in a variety of antibody-dependent experimental procedures. Expression vectors producing epitope tag fusion proteins are available for a variety of host expression systems including bacteria, yeast, insect and mammalian cells.