HA-Tag antibody (Biotin)

Catalog No. ABIN401230

Product Details anti-HA-Tag Antibody (Biotin)

Target: HA-Tag

Reactivity: Please inquire

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HA-Tag antibody is conjugated to Biotin

Application: Western Blotting (WB), Immunoprecipitation (IP), Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Sequence: YPYDVPDYA

Characteristics: Molar Ratio: 10-20 BAC molecules per Rabbit IgG molecule

Purification: Affinity chromatography

Immunogen: 9-aa epitope tag peptide YPYDVPDYA (11 114-122) from hemagglutinin influenza conjugated to KLH using maleimide

Isotype: IgG

Application Details

Application Notes: ELISA: 1: 15,000 - 1: 150,000. Immunohistochemistry on paraffin sections: 1: 250. Immunoprecipitation: 1: 250. Western blot: 1: 10,000 (In western blotting of bacterial extracts the antibody does notcross-react with endogenous proteins).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore with 0.1 mL of deionized water (or equivalent).

Concentration: 1.0 mg/mL (by UV absorbance at 280 nm)

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) IgG and Protease free, 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing. Centrifuge product if not completely clear after standing at room temperature.

Storage: 4 °C/-20 °C

Storage Comment: Store vial at 2-8 ° C prior to restoration. Following restoration product can be stored undiluted at 2-8 ° for up to one month or (in aliquots) at -20 °C or below. For extended storage add glycerol to 50%.

Target Details for HA-Tag

Target: HA-Tag

Alternative Name: HA Epitope Tag (YPYDVPDYA) (HA-Tag Products)

Target Type: Tag

Background: Epitope tags are short peptide sequences that are easily recognized by tag-specific antibodies. Due to their small size, epitope tags do not affect the tagged protein's biochemical properties. Most often sequences encoding the epitope tag are included with target DNA at the time of cloning to produce fusion proteins containing the epitope tag sequence. This allows anti-epitope tag antibodies to serve as universal detection reagents for any tag containing protein produced by recombinant means. This means that anti-epitope tag antibodies are a useful alternative to generating specific antibodies to identify, immunoprecipitate or immunoaffinity purify a recombinant protein. The anti-epitope tag antibody is usually functional in a variety of antibody-dependent experimental procedures.