RFP antibody (Biotin)

Catalog No. ABIN401226

Product Details anti-RFP Antibody (Biotin)

Target: RFP (Red Fluorescent Protein (RFP))

Reactivity: Discosoma

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RFP antibody is conjugated to Biotin

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Frozen Sections) (IHC (fro))

Cross-Reactivity (Details): Ads. to Hu, Ms, Rt Serum Proteins

Purification: Immunoaffinity Chromatography using Red Fluorescent Protein (Discosoma) coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.

Immunogen: Red Fluorescent Protein (RFP) fusion protein corresponding to the full length amino acid sequence (234aa) derived from the mushroom polyp coral Discosoma

Isotype: IgG

Application Details

Application Notes: Polyclonal anti-RFP is designed to detect RFP and its variants. This antibody can be used todetect RFP by ELISA (sandwich or capture) for the direct binding of antigen. Biotinconjugated polyclonal anti-RFP used in a sandwich ELISA with unconjugated anti-RFP iswell suited to titrate RFP in solution. The detection antibody conjugated to biotin issubsequently reacted with streptavidin conjugated HRP. Fluorochrome conjugatedpolyclonal anti-RFP can be used to detect RFP by immunofluorescence microscopy in cellexpression systems and can detect RFP containing inserts. Significant amplification ofsignal is achieved using fluorochrome conjugated polyclonal anti-RFP relative to thefluorescence of RFP alone. For immunoblotting use either alkaline phosphatase orperoxidase conjugated polyclonal anti-RFP to detect RFP or RFP containing proteins onwestern blots. Recommended Dilutions: ELISA: 1/10,000-1/50,000. Western blot: 1/2,000-1/10,000. Immunohistochemistry on Frozen Sections.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Comment:

Molar Ratio: 10-20 BAC molecules per IgG molecule

Restrictions: For Research Use only

Handling

Reconstitution: Restore with 0.1 mL of deionized water (or equivalent).

Concentration: 1.0 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.12 M Sodium Chloride, pH 7.2, 0.01 % (w/v) Sodium Azide, 10 mg/mL BSA (IgG and Protease free)

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Prior to reconstitution store at 2-8 °C. Following reconstitution store undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

Target Details for RFP

Target: RFP (Red Fluorescent Protein (RFP))

Abstract: RFP Products

Background: Epitope tagging is a powerful and versatile strategy for detecting and purifying proteins expressed by cloned genes. To utilize this feature, protein expression vectors are typically engineered with a nucleotide sequence that encodes the peptide epitope tag. The gene of interest is cloned in-frame relative to the tag and, upon expression, the protein of interest is synthesized as a fusion protein with the peptide tag. Fusion protein detection and/or purification is mediated by highly specific antibodies to the engineered peptide, thus eliminating the need for antibodies to proteins from each newly cloned gene. Commonly used epitope tags include glutathione-S-transferase (GST), c-myc, 6-histidine (6X-His), FLAG, green fluorescent protein (GFP), red fluorescent protein (RFP, DSRed), maltose binding protein (MBP), influenza A virus haemagglutinin (HA), b-galactosidase, and GAL4.Synonyms: DsRed Tag, Red fluorescent protein Tag

Gene ID: 86600

UniProt: Q9U6Y8