Albumin antibody

Catalog No. ABIN401206

Product Details anti-Albumin Antibody

Target: Albumin (ALB)

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Albumin antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro))

Specificity: This antoibody detects Serum Albumin.

Cross-Reactivity (Details): Species reactivity (tested):Mouse.

Purification: Protein A Sepharose Fast Flow followed by POROS EP antigen resin affinity purification, dialyzed against PBS with sodium azide, concentrated by Amicon ultrafiltration, 0.2 μm filtered, aseptically filled

Immunogen: Mouse Serum Albumin

Isotype: IgG

Application Details

Application Notes: ELISA: 1: 10,000 to 1: 50,000 (1.0 μg of Mouse Serum Albumin in a standard sandwich ELISAusing Peroxidase conjugated Affinity Purified Donkey anti-Rabbit IgG [H&L] MX and TMB asa substrate for 30 minutes at room temperature.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL (by UV absorbance at 280 nm)

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing. Should this product contain a precipitate we recommend microcentrifugation before use.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody at 2 - 8 °C up to one month or for longer (in aliquots) at -20 °C or below.

Target Details for Albumin

Target: Albumin (ALB)

Alternative Name: Albumin (ALB Products)

Background: Synonyms: ALB, BSA, HSA, Serum Albumin

Gene ID: 11657

NCBI Accession: NP_033784

UniProt: P07724

Pathways: Lipid Metabolism