beta Amyloid antibody (N-Term)

Catalog No. ABIN401205

Product Details anti-beta Amyloid Antibody

Target: beta Amyloid (Abeta) (Amyloid beta (Abeta))

Binding Specificity: AA 1-14, N-Term

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This beta Amyloid antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Sequence: D-A-E-F-R-H-D-S-G-Y-E-V-H-H

Cross-Reactivity (Details): Species reactivity (tested):Human, Mouse.

Purification: Affinity Chromatography

Immunogen: Synthetic peptide corresponding to the amino terminus (1-14) of Human beta amyloid conjugated to KLH using maleimide.

Isotype: IgG

Application Details

Application Notes: ELISA: 1/10,000. Western blot: 0.5-1.0 μg/mL. A 40-50 kD band consistent with a higher MW precursor isdetected in using whole tissue extracts from mouse brain. Immunofluorescence. Immunohistochemistry: We recommend the use of 4 % PFA for Paraffin embedded tissuesand 10 % Formalin for Frozen tissue for fixation.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.55 mg/mL (by UV absorbance at 280 nm)

Buffer: 0.02 M Potassium Phosphate, 0.12 M Sodium Chloride, pH 7.2, 0.01 % (w/v) Sodium Azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing. Should this product contain a precipitate we recommend microcentrifugation before use.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or(in aliquots) at-20 °C or below for longer .

Target Details for beta Amyloid

Target: beta Amyloid (Abeta) (Amyloid beta (Abeta))

Alternative Name: Amyloid beta (Abeta Products)

Background: Beta amyloid, often abbreviated as A-beta, is a protein that builds up in the brains of persons with Alzheimer's disease, collecting in clumps called plaques or senile plaques. While some researchers question whether beta amyloid is the cause of the dementia, most agree that it is involved in the disruption of thinking that is a hallmark of the disease. In some cases of familial Alzheimer's disease, mutations in genes for the proteins called the presenilins lead to increased production of amyloid. Researchers have been looking at how presenilin-1 in particular contributes to the excess buildup of beta amyloid. Presenilin-1 apparently acts to increase the activity of gamma-secretase, an enzyme that changes a normal protein (amyloid precursor protein or APP) into beta amyloid itself. Furthermore, presenilin-1 might be gamma-secretase.Synonyms: Amyloid beta peptide

Pathways: Inflammasome