GFP antibody

Catalog No. ABIN401203

Product Details anti-GFP Antibody

Target: GFP (Green Fluorescent Protein (GFP))

Reactivity: Aequorea victoria

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GFP antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA)

Cross-Reactivity (Details): Species reactivity (tested):A. Victoria
Ads. to Hu, Ms, Rt Serum Proteins

Purification: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Green Fluorescent Protein (Aequorea victoria) coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum and purified and partially purified Green Fluorescent Protein (Aequorea victoria). No reaction was observed against Human, Mouse or Rat serum proteins.

Immunogen: Green Fluorescent Protein (GFP) fusion protein corresponding to the full length amino acid sequence (246aa) derived from the jellyfish Aequorea victoria

Isotype: IgG

Application Details

Application Notes: Immunohistochemsitry on frozen section: 1: 200 - 1: 3,000. Western blot: 1: 500 - 1: 5,000. ELISA.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.6 mg/mL (by UV absorbance at 280 nm)

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Handling Advice: Avoid repeated freezing and thawing. Should this product contain a precipitate we recommend microcentrifugation before use.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer.

References for anti-GFP antibody (ABIN401203)

Choi, Tian, Manohar, Harraz, Park, Schroeder, Snyder, Klessig: "Human GAPDH Is a Target of Aspirin's Primary Metabolite Salicylic Acid and Its Derivatives." in: PLoS ONE, Vol. 10, Issue 11, pp. e0143447, (2016) (PubMed).

Jiang, Wang, Wang, Hawke, Zheng, Li, Zhou, Majumder, Bi, Liu, Huang, Lu: "PKM2 phosphorylates MLC2 and regulates cytokinesis of tumour cells." in: Nature communications, Vol. 5, pp. 5566, (2015) (PubMed).

Navarro, Botella, Metzendorf, Lind, Schneuwly: "Mitoferrin modulates iron toxicity in a Drosophila model of Friedreich?s ataxia." in: Free radical biology & medicine, Vol. 85, pp. 71-82, (2015) (PubMed).

Takeda, Jain, Leboeuf, Padmanabhan, Wang, Li, Lu, Millar, Epstein: "Hopx expression defines a subset of multipotent hair follicle stem cells and a progenitor population primed to give rise to K6+ niche cells." in: Development (Cambridge, England), Vol. 140, Issue 8, pp. 1655-64, (2013) (PubMed).

Venters, Cuenca, Hyer: "Retinal and anterior eye compartments derive from a common progenitor pool in the avian optic cup." in: Molecular vision, Vol. 17, pp. 3347-63, (2012) (PubMed).

Papadopoulos, Arato, Lilienthal, Zerweck, Schutkowski, Chatain, Müller-Newen, Becker, de la Luna: "Splice variants of the dual specificity tyrosine phosphorylation-regulated kinase 4 (DYRK4) differ in their subcellular localization and catalytic activity." in: The Journal of biological chemistry, Vol. 286, Issue 7, pp. 5494-505, (2011) (PubMed).

Torres, Funk, Zegers, ter Beest: "The syntaxin 4 N terminus regulates its basolateral targeting by munc18c-dependent and -independent mechanisms." in: The Journal of biological chemistry, Vol. 286, Issue 12, pp. 10834-46, (2011) (PubMed).

Schmidt, Bleck, Helenius, Mercer: "Vaccinia extracellular virions enter cells by macropinocytosis and acid-activated membrane rupture." in: The EMBO journal, Vol. 30, Issue 17, pp. 3647-61, (2011) (PubMed).

Kwan, Navarro-Sanchez, Dumortier, Decossas, Vachon, dos Santos, Fridman, Rey, Harris, Despres, Mueller: "Dermal-type macrophages expressing CD209/DC-SIGN show inherent resistance to dengue virus growth." in: PLoS neglected tropical diseases, Vol. 2, Issue 10, pp. e311, (2008) (PubMed).

Target Details for GFP

Target: GFP (Green Fluorescent Protein (GFP))

Alternative Name: GFP (GFP Products)

Background: Green fluorescence protein (GFP) is a 27 kDa protein derived from the jellyfish Aequorea victoria, which emits green light (emission peak at a wavelenth of 509 nm) when excited by blue light (excitation peak at a wavelenth of 395 nm). Green Fluorescent Protein (GFP) has become an invaluable tool in cell biology research, since its intrinsic fluorescence can be visualized in living cells. GFP fluorescence is stable under fixation conditions and suitable for a variety of applications. GFP has been widely used as a reporter for gene expression, enabling researchers to visualize and localize GFP-tagged proteins within living cells without the need for chemical staining. Other applications of GFP include assessment of protein protein interactions through the yeast two hybrid system and measurement of distance between proteins through fluorescence energy transfer (FRET) protocols. GFP technnology has considerably contributed to a greater understanding of cellular physiology.Synonyms: GFP-Tag, Green fluorescent protein

Gene ID: 6100

UniProt: P42212