gamma 1 Adaptin antibody (AA 646-659)

Catalog No. ABIN401169

Product Details anti-gamma 1 Adaptin Antibody

Target: gamma 1 Adaptin (AP1G1) (Adaptor-Related Protein Complex 1, gamma 1 Subunit (AP1G1))

Binding Specificity: AA 646-659

Reactivity: Human, Chimpanzee, Orang-Utan

Host: Goat

Clonality: Polyclonal

Conjugate: This gamma 1 Adaptin antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Specificity: This antibody is directed against AP1G1.

Cross-Reactivity (Details): Species reactivity (expected):Chimpanzee, Orangutan, Mouse, Rat, Canine, Chicken.
Species reactivity (tested):Human.

Purification: Affinity chromatography

Immunogen: Synthetic peptide corresponding aa 646-659 of Human AP1G1

Isotype: IgG

Application Details

Application Notes: ELISA: 1/2,000 - 1/8,000. Western Blot: 1/500 - 1/2,000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.7 mg/mL (by UV absorbance at 280 nm)

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Target Details for gamma 1 Adaptin

Target: gamma 1 Adaptin (AP1G1) (Adaptor-Related Protein Complex 1, gamma 1 Subunit (AP1G1))

Alternative Name: AP1 Complex Subunit gamma-1 (AP1G1 Products)

Background: AP1G1 (also known as gamma 1 subunit of adaptor -related protein complex 1, Gammaadaptin, Adaptor protein complex AP-1 gamma-1 subunit, Golgi adaptor HA1/AP1 adaptin gamma-1 subunit and Clathrin assembly protein complex 1 gamma-1 large chain) is a subunit of clathrin-associated adaptor protein complex 1 that plays a role in protein sorting in the late- Golgi/trans-Golgi network (TGN) and/or endosomes. The AP complexes mediate both the recruitment of clathrin to membranes and the recognition of sorting signals within the cytosolic tails of transmembrane cargo molecules. The adaptin family of proteins is composed of four classes of molecules named alpha, beta-, beta prime- and gamma- adaptins. Adaptins, together with medium and small subunits, form a heterotetrameric complex called an adaptor, whose role is to promote the formation of clathrin-coated pits and vesicles. AP1G1 is localized to the coat surrounding the cytoplasmic face of coated vesicles located at the Golgi complex and is widely expressed.Synonyms: ADTG, AP1G1, Adapter-related protein complex 1 subunit gamma-1, Adaptor protein complex AP-1 subunit gamma-1, CLAPG1, Clathrin assembly protein complex 1 gamma-1 large chain, Golgi adaptor HA1/AP1, gamma 1 Adaptin, gamma Adaptin

Gene ID: 164

NCBI Accession: NP_001025178

UniProt: O43747