IL32 antibody (Isoform 4)

Catalog No. ABIN401103

Product Details anti-IL32 Antibody

Target: IL32 (Interleukin 32 (IL32))

Binding Specificity: Isoform 4

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IL32 antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA), Neutralization (Neut)

Specificity: This antibody will recognize both native and recombinant Human IL-32A in cell supernatants and certain body fluids. A control of similarly diluted normal rabbit IgG is recommended.

Purification: Ig Fraction

Immunogen: Full length recombinant Human IL-32A protein

Isotype: IgG

Application Details

Application Notes: ELISA: 1/20,000 - 1/100,000. Western Blot: 1/500 - 1/2,000. Neutralization: 1/400.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore with 0.1 mL of deionized water (or equivalent).

Concentration: 1.0 mg/mL by UV absorbance at 280 nm

Buffer: 0.02 M Potassium Phosphate, 0.12 M Sodium Chloride, pH 7.2 without preservatives.

Preservative: Without preservative

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Prior to reconstitution store at 2-8 °C. Following reconstitution store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

Target Details for IL32

Target: IL32 (Interleukin 32 (IL32))

Alternative Name: Interleukin-32 / IL32 (IL32 Products)

Synonyms: IL-32alpha antibody, IL-32beta antibody, IL-32delta antibody, IL-32gamma antibody, NK4 antibody, TAIF antibody, TAIFa antibody, TAIFb antibody, TAIFc antibody, TAIFd antibody, interleukin 32 antibody, IL32 antibody

Background: IL-32A (also known as Natural killer cells protein 4, Tumor necrosis factor alpha-inducing factor, IL32a, Interleukin-32 and IL-32 isoform 4) is a member of the cytokine family. IL-32a is a secreted protein selectively expressed in lymphocytes and plays a role in innate and adaptive immune responses. The protein contains a tyrosine sulfation site, 3 potential N-myristoylation sites, multiple putative phosphorylation sites, and an RGD cell-attachment sequence. Expression of this protein is increased after the activation of T-cells by mitogens or the activation of NK cells by IL-2. This protein induces the production of TNFa and IL-8. It induces typical cytokine pathways of NF-kB and p38 MAPK. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.Synonyms: IL-32, NK4, Natural killer cells protein 4, TAIF, Tumor necrosis factor alpha-inducing factor

Gene ID: 9235

NCBI Accession: NP_001012649

UniProt: P24001