RFFL antibody (N-Term)

Catalog No. ABIN401034

Product Details anti-RFFL Antibody

Target: RFFL (Ring Finger and FYVE-Like Domain Containing 1 (RFFL))

Binding Specificity: AA 1-363, N-Term

Reactivity: Human, Mouse, Rat, Cow, Dog

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RFFL antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Specificity: This antibody is directed against RFFL protein.

Cross-Reactivity (Details): Species reactivity (expected):Canine, Rat, Mouse, Bovine.
Species reactivity (tested):Human.

Purification: Affinity chromatography on Protein A

Immunogen: Recombinant protein corresponding to amino acids 1-363 of human RFFL protein

Isotype: IgG

Application Details

Application Notes: ELISA: 1/4000 - 1/20000. Western Blot: 1/500 - 1/3000. Immunohistochemistry: 1/500 - 1/3000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore with 0.1 mL of deionized water or equivalent.

Concentration: 5.0 mg/mL (by UV absorbance at 280 nm)

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

Target Details for RFFL

Target: RFFL (Ring Finger and FYVE-Like Domain Containing 1 (RFFL))

Alternative Name: RFFL / RNF189 (RFFL Products)

Background: RFFL, also known as Rififylin, RING finger and FYVE-like domaincontaining protein 1, FYVE-RING finger protein, Sakura, Fring, Caspases-8 and -10-associated RING finger protein 2, CARP-2, Caspase regulator CARP2, RING finger protein 189 and RING finger protein 34-like, is a novel modulator of NF-kappaB activation. RFFL possesses E3 ubiquitin protein ligase activity and has been shown to regulate the levels of CASP8 and CASP10 by targeting them for proteasomal degradation. RFFL also possesses anti-apoptotic activity and may bind phosphatidylinositol phosphates. RFFL is a membrane bound cytoplasmic protein that is expressed ubiquitously. RFFL can be detected in spleen, thymus, prostate, testis, ovary, small intestine, colon and peripheral blood leukocytes and is rapidly degraded after stimulation with TNFSF10, and probably by caspases. Multiple transcript variants have been detected for this protein.Synonyms: CARP-2, CARP2, Caspases-8 and -10-associated RING finger protein 2, E3 ubiquitin-protein ligase rififylin, FYVE-RING finger protein Sakura, Fring, RIFIFYLIN, RING finger protein 189, RING finger protein 34-like, RNF34L

Gene ID: 117584

NCBI Accession: NP_001017368

UniProt: Q8WZ73