NRF1 antibody (N-Term)

Catalog No. ABIN401025

Product Details anti-NRF1 Antibody

Target: NRF1 (Nuclear Respiratory Factor 1 (NRF1))

Binding Specificity: AA 1-534, N-Term

Reactivity: Human, Mouse, Rat, Zebrafish (Danio rerio), Chicken, Frog, Chimpanzee

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NRF1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Cross-Reactivity (Details): Species reactivity (expected):Human, Chimpanzee, Canine, Rat, Chicken, Frog, Zebrafish.
Species reactivity (tested):Mouse.

Purification: Affinity Chromatography on Protein A.

Immunogen: Recombinant Mouse NRF1 protein corresponding to aa 1-534 of the native protein. Genename: NRF1

Isotype: IgG

Application Details

Application Notes: ELISA: 1/3000-1/10000. Western Blot: 1/500-1/2000. Immunohistochemistry on Paraffin Sections: 2 mg/mL-5 μg/mL. Expect a band approx. 67 kDa in size corresponding to NRF1 by Western blotting in theappropriate cell lysate or extract. Splice variants exist for this protein that may result in the detection of Lower MolecularWeight bands.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 2.3 mg/mL (by UV absorbance at 280 nm)

Buffer: 0.02 M Potassium Phosphate, 0.12 M Sodium Chloride, pH 7.2 containing 0.01 % (w/v) Sodium Azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store the antibody (in aliquots) at-20 °C. Dilute only prior to immediate use.

Target Details for NRF1

Target: NRF1 (Nuclear Respiratory Factor 1 (NRF1))

Alternative Name: NRF1 (NRF1 Products)

Background: NRF1 (also known as nuclear respiratory factor 1, alpha palindromic binding protein and alpha-pal) is the mammalian homolog to the erect wing (ewg) Drosophila protein that is required for proper development of the central nervous system and indirect flight muscles. In mammals NRF1 functions as a transcription factor that activates the expression of the EIF2S1 (EIF2-alpha) gene. This protein links the transcriptional modulation of key metabolic genes to cellular growth and development and has been implicated in the control of nuclear genes required for respiration, heme biosynthesis, and mitochondrial DNA transcription and replication. NRF1 forms a homodimer and binds DNA as a dimer. NRF1 shows a nuclear localization and is widely expressed in embryonic, fetal, and adult tissues. Phosphorylation of NRF1 enhances DNA binding. Multiple splice variants have been identified for this protein.Synonyms: Alpha palindromic-binding protein, Alpha-pal, NRF-1, Nuclear respiratory factor 1

Gene ID: 10090

UniProt: Q9WU00

Pathways: Regulation of Lipid Metabolism by PPARalpha