SELPLG antibody

Catalog No. ABIN400881

Product Details anti-SELPLG Antibody

Target: SELPLG (Selectin P Ligand (SELPLG))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This SELPLG antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Specificity: The antibody TC2 reacts with CD162, a 220 kDa type I integral membrane protein expressed as disulfide-linked homodimer (sialomucin family). CD162 is present on the most peripheral blood T lymphocytes, monocytes, granulocytes, it is also expressed on a subpopulation of B lymphocytes and CD34+ bone marrow cells.

Cross-Reactivity (Details): Species reactivity (tested):Human.

Characteristics: Synonyms: PSGL-1, Selectin P ligand, SELPLG, P-selectin glycoprotein ligand 1

Purification: Protein A affinity chromatography

Purity: > 95 % by SDS-PAGE

Clone: TC2

Isotype: IgG1

Application Details

Application Notes: Flow cytometry: 1.5 - 2.5 μg/mL. Positive control: human peripheral blood.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS, pH ~7.4, 15 mM Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C. Avoid repeated freezing andthawing.
Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for SELPLG

Target: SELPLG (Selectin P Ligand (SELPLG))

Alternative Name: CD162 / PSGL1 (SELPLG Products)

Background: CD162 (P-selectin glycoprotein ligand-1, PSGL-1) is a sialomucin constitutively expressed as a disulfide-linked homodimer of two 120 kDa subunits on the surface of circulating leukocytes. CD162 serves as a ligand for P- E- and L-selectin, with the highest affinity for P-selectin. It is thus involved in leukocyte rolling at the endothelial surfaces, prerequisite for firm leukocyte adhesion and subsequent transendothelial migration. CD162 also mediates leukocyte-platelet adhesion and interleukocyte contacts. Whereas serving as an adhession molecule on mature leukocytes, CD162 is a potent negative regulator of human hematopoietic progenitors.Synonyms: P-selectin glycoprotein ligand 1, PSGL-1, SELPLG, Selectin P ligand

Gene ID: 6404

UniProt: Q14242