TRPC6 antibody (C-Term)

Catalog No. ABIN400824

Product Details anti-TRPC6 Antibody

Target: TRPC6 (Transient Receptor Potential Cation Channel, Subfamily C, Member 6 (TRPC6))

Binding Specificity: C-Term

Reactivity: Human, Chimpanzee

Host: Mouse

Clonality: Monoclonal

Conjugate: This TRPC6 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Specificity: This antibody is specific for Human TRPC6 protein.

Cross-Reactivity (Details): Species reactivity (expected):Chimpanzee.
Species reactivity (tested):Human.

Characteristics: Synonyms: TRP-6, Short transient receptor potential channel 6

Purification: Affinity Chromatography on Protein A

Immunogen: Synthetic peptide corresponding to a region near the carboxy terminus of human TRPC6protein.

Clone: 3F2-H10-F2

Isotype: IgG1

Application Details

Application Notes: Immunohistochemistry: 2.5 μg/mL. Western Blot: 1/500- 1/2000. ELISA: 1/10000 - 1/50000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL (by UV absorbance at 280 nm)

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for TRPC6

Target: TRPC6 (Transient Receptor Potential Cation Channel, Subfamily C, Member 6 (TRPC6))

Alternative Name: TRPC6 (TRPC6 Products)

Background: TRPC6, also known as TRP6, short transient receptor potential channel 6 and transient receptor potential cation channel subfamily C member 6, is thought to form a receptor-activated non-selective calcium permeant cation channel. TRPC6 is probably operated by a phosphatidylinositol second messenger system activated by receptor tyrosine kinases or Gprotein coupled receptors. It is activated by diacylglycerol (DAG) in a membrane-delimited fashion, independently of protein kinase C and may not to be activated by intracellular calcium store depletion. Defects in this gene are a cause of focal segmental glomerulosclerosis (FSGS). Expression of this protein has been reported in tissues such as placenta, lung, spleen, ovary, small intestine, and renal podocytes. Immunohistochemistry studies using polyclonal antibodies to this target have shown moderate to strong staining in cell types such as neurons, breast, respiratory, squamous and prostate epithelium, epidermis, placental trophoblasts, dendritic cells, and subsets of immune cells, and faint to moderate staining of adrenal, colon, ganglion cells, hepatocytes, heart, and testis.Synonyms: Short transient receptor potential channel 6, TRP-6, Transient receptor protein 6

Gene ID: 7225

UniProt: Q9Y210